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Literature summary for 3.2.1.67 extracted from

  • Kobayashi, T.; Higaki, N.; Yajima, N.; Suzumatsu, A.; Hagihara, H.; Kawai, S.; Ito, S.
    Purification and properties of a galacturonic acid-releasing exopolygalacturonase from a strain of Bacillus (2001), Biosci. Biotechnol. Biochem., 65, 842-847.
    View publication on PubMed
Search for more literature for 3.2.1.67

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 0.5 mM, 40-50% inhibition Bacillus sp. (in: Bacteria)
EDTA 10 mM, 10% inhibition Bacillus sp. (in: Bacteria)
N-bromosuccinimide 0.5 mM, 67% inhibition Bacillus sp. (in: Bacteria)
Ni2+ 0.5 mM, 40-50% inhibition Bacillus sp. (in: Bacteria)
p-chloromercuribenzoate 0.5 mM, 29% inhibition Bacillus sp. (in: Bacteria)
Pd2+ 0.5 mM, 40-50% inhibition Bacillus sp. (in: Bacteria)
Zn2+ 0.5 mM, 40-50% inhibition Bacillus sp. (in: Bacteria)

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Bacillus sp. (in: Bacteria)
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no requirement for metal ions Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
 KSM-P443
-
Bacillus sp. (in: Bacteria) KSM-P443
-
 KSM-P443
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
54
-
 pH 6.8, 30°C Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(1,4-alpha-D-galacturonide)n + H2O
-
 Bacillus sp. (in: Bacteria) D-galacturonic acid
-
 ?
(1,4-alpha-D-galacturonide)n + H2O
-
 Bacillus sp. (in: Bacteria) KSM-P443 D-galacturonic acid
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 stable up to Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Bacillus sp. (in: Bacteria)

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 11.5
-
 Bacillus sp. (in: Bacteria)