Activating Compound | Comment | Organism | Structure |
---|---|---|---|
HgCl2 | 0.01 mM, 3.4fold increase in activity, exo-PG2, no effect with exo-PG1 | Aspergillus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
x * 82000, exo-PG1, x * 56000, exo-PG2, SDS-PAGE | Aspergillus niger |
82000 | - |
x * 82000, exo-PG1, x * 56000, exo-PG2, SDS-PAGE | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
two isozymes | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | molecular mass after deglycosilation: 71000, exo-PG1, 43000, exo-PG2, deglycosylated proteins show about 50% residual activity | Aspergillus niger |
Purification (Comment) | Organism |
---|---|
- |
Aspergillus niger |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
13.5 | - |
exo-PG1, pH 4.0, 40°C | Aspergillus niger |
31.4 | - |
exo-PG2, pH 4.0, 40°C | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(1,4-alpha-D-galacturonide)n + H2O | - |
Aspergillus niger | D-galacturonic acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 82000, exo-PG1, x * 56000, exo-PG2, SDS-PAGE | Aspergillus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Aspergillus niger |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
30 min, 75% residual activity, exo-PG1, 25% residual activity, exo-PG2 | Aspergillus niger |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.4 | 3.8 | exo-PG1 | Aspergillus niger |
3.4 | 4.2 | exo-PG2 | Aspergillus niger |