Literature summary for 3.2.1.65 extracted from

Porras-Dominguez, J.R.; Rodriguez-Alegria, M.E.; Avila-Fernandez, A.; Montiel-Salgado, S.; Lopez-Munguia, A.
Levan-type fructooligosaccharides synthesis by a levansucrase-endolevanase fusion enzyme (LevB1SacB) (2017), Carbohydr. Polym., 177, 40-48 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli Rosetta 2 cells	Bacillus licheniformis

Organism

Organism	UniProt	Comment	Textmining
Bacillus licheniformis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
HiTrap column chromatography	Bacillus licheniformis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
high molecular weight levan + H2O	-	Bacillus licheniformis	D-fructose + fructooligosaccharides DP2-10	-	?
low molecular weight levan + H2O	-	Bacillus licheniformis	D-fructose + fructooligosaccharides DP2-10	-	?
additional information	the levansucrase-endolevanase fusion enzyme LevB1SacB or both enzymes (levanase LevB1 and levansucrase SacB) at equimolar concentrations in simultaneous reactions result in an optimal, wide and diverse L-FOS profile, including 6-kestose, levanbiose and blastose among other L-FOS and 1-kestose, which accumulates as by-product of SacB levan synthesis	Bacillus licheniformis	?	-	?

Synonyms

Synonyms	Comment	Organism
endolevanase	-	Bacillus licheniformis
LevB1	-	Bacillus licheniformis

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Release 2023.1 (January 2023)