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Literature summary for 3.2.1.65 extracted from

  • Menendez, C.; Hernandez, L.; Selman, G.; Mendoza, M.F.; Hevia, P.; Sotolongo, M.; Arrieta, J.G.
    Molecular cloning and expression in Escherichia coli of an exo-levanase gene from the endophytic bacterium Gluconacetobacter diazotrophicus SRT4 (2002), Curr. Microbiol., 45, 5-12.
    View publication on PubMed
Search for more literature for 3.2.1.65

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Gluconacetobacter diazotrophicus

Inhibitors

Inhibitors Comment Organism Structure
CuSO4 1 mM, pH 6.0, 1% levan, for 6 h at 30ºC, 27% inhibition Gluconacetobacter diazotrophicus
additional information not inhibited by EDTA, dithiothreitol and beta-mercaptoethanol Gluconacetobacter diazotrophicus
SDS 1%, pH 6.0, 1% levan, for 6 h at 30ºC, 97% inhibition Gluconacetobacter diazotrophicus

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Gluconacetobacter diazotrophicus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information not significantly influenced by 1 mM Ca2+, Mg2+, or Fe3+, at 30ºC, pH 6.0, 1% levan, for 6 h Gluconacetobacter diazotrophicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 SDS-PAGE Gluconacetobacter diazotrophicus
58400
-
 calculation from sequence of cDNA Gluconacetobacter diazotrophicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
levan + H2O Gluconacetobacter diazotrophicus
-
 fructose + beta-1,2-oligofructans
-
 ?
levan + H2O Gluconacetobacter diazotrophicus SRT4
-
 fructose + beta-1,2-oligofructans
-
 ?

Organism

Organism UniProt Comment Textmining
Gluconacetobacter diazotrophicus Q43998
-
-
Gluconacetobacter diazotrophicus SRT4 Q43998
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no modification
-
 Gluconacetobacter diazotrophicus

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 Gluconacetobacter diazotrophicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-kestose + H2O
-
 Gluconacetobacter diazotrophicus D-fructose + sucrose
-
 ?
1-kestose + H2O
-
 Gluconacetobacter diazotrophicus SRT4 D-fructose + sucrose
-
 ?
D(+)-raffinose + H2O
-
 Gluconacetobacter diazotrophicus D-fructose + ?
-
 ?
inulin + H2O without formation of intermediate oligofructans Gluconacetobacter diazotrophicus D-fructose + ?
-
 ?
inulin + H2O without formation of intermediate oligofructans Gluconacetobacter diazotrophicus SRT4 D-fructose + ?
-
 ?
levan + H2O 4fold higher rate of hydrolysis compared to inulin, without formation of intermediate oligofructans, no activity with D(+)-melezitose Gluconacetobacter diazotrophicus D-fructose + ?
-
 ?
levan + H2O 4fold higher rate of hydrolysis compared to inulin, without formation of intermediate oligofructans, no activity with D(+)-melezitose Gluconacetobacter diazotrophicus SRT4 D-fructose + ?
-
 ?
levan + H2O
-
 Gluconacetobacter diazotrophicus fructose + beta-1,2-oligofructans
-
 ?
levan + H2O
-
 Gluconacetobacter diazotrophicus SRT4 fructose + beta-1,2-oligofructans
-
 ?
sucrose + H2O
-
 Gluconacetobacter diazotrophicus D-fructose + D-glucose
-
 ?
sucrose + H2O
-
 Gluconacetobacter diazotrophicus SRT4 D-fructose + D-glucose
-
 ?

Synonyms

Synonyms Comment Organism
exo-levanase
-
 Gluconacetobacter diazotrophicus
LsdB
-
 Gluconacetobacter diazotrophicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Gluconacetobacter diazotrophicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 irreversibly inactivated above Gluconacetobacter diazotrophicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Gluconacetobacter diazotrophicus

pH Range

pH Minimum pH Maximum Comment Organism
4 7 inactive below pH 4.0, decreased activity above pH 7.0 Gluconacetobacter diazotrophicus

pI Value

Organism Comment pI Value Maximum pI Value
Gluconacetobacter diazotrophicus pH-gradient 4.0-8.0
-
 6.5