Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Tannerella forsythia |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
deoxyfuconojirimycin | complete inhibition at 0.0001 mM | Tannerella forsythia | |
L-fucose | complete inhibition at 0.25 mM | Tannerella forsythia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.67 | - |
4-nitrophenyl alpha-L-fucopyranoside | at pH 9.0 and 22°C | Tannerella forsythia |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Tannerella forsythia | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tannerella forsythia | - |
- |
- |
Tannerella forsythia ATCC 43037 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA bead chromatography | Tannerella forsythia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-fucosyllactose + H2O | - |
Tannerella forsythia | L-fucose + lactose | - |
? | |
2'-fucosyllactose + H2O | - |
Tannerella forsythia ATCC 43037 | L-fucose + lactose | - |
? | |
4-nitrophenyl alpha-L-fucopyranoside + H2O | - |
Tannerella forsythia | 4-nitrophenol + L-fucopyranose | - |
? | |
4-nitrophenyl alpha-L-fucopyranoside + H2O | - |
Tannerella forsythia ATCC 43037 | 4-nitrophenol + L-fucopyranose | - |
? | |
alpha-L-Fuc-(1->6)-beta-D-GlcNAc + H2O | the enzyme also possesses alpha(1,6) specificity on small unbranched substrates | Tannerella forsythia | ? | - |
? | |
alpha-L-Fuc-(1->6)-beta-D-GlcNAc + H2O | the enzyme also possesses alpha(1,6) specificity on small unbranched substrates | Tannerella forsythia ATCC 43037 | ? | - |
? | |
bovine submaxillary mucin + H2O | - |
Tannerella forsythia | L-fucose + ? | - |
? | |
bovine submaxillary mucin + H2O | - |
Tannerella forsythia ATCC 43037 | L-fucose + ? | - |
? | |
additional information | the alpha(1,3) and alpha(1,4) linkages are not cleaved on 3-fucosyllactose and the Lewis A trisaccharide, respectively. The enzyme is also inactive on A antigen tetraose and alpha-L-Fuc-(1->4)-beta-D-Gal | Tannerella forsythia | ? | - |
? | |
additional information | the alpha(1,3) and alpha(1,4) linkages are not cleaved on 3-fucosyllactose and the Lewis A trisaccharide, respectively. The enzyme is also inactive on A antigen tetraose and alpha-L-Fuc-(1->4)-beta-D-Gal | Tannerella forsythia ATCC 43037 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 51000, SDS-PAGE | Tannerella forsythia |
Synonyms | Comment | Organism |
---|---|---|
alpha(1,2) fucosidase | - |
Tannerella forsythia |
bfo_2737 | - |
Tannerella forsythia |
Fuc1 | - |
Tannerella forsythia |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Tannerella forsythia |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme contributes to the virulence of Tannerella forsythia | Tannerella forsythia |