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Literature summary for 3.2.1.63 extracted from

  • Wada, J.; Honda, Y.; Nagae, M.; Kato, R.; Wakatsuki, S.; Katayama, T.; Taniguchi, H.; Kumagai, H.; Kitaoka, M.; Yamamoto, K.
    1,2-alpha-l-Fucosynthase: a glycosynthase derived from an inverting alpha-glycosidase with an unusual reaction mechanism (2008), FEBS Lett., 582, 3739-3743.
    View publication on PubMed
Search for more literature for 3.2.1.63

Application

Application Comment Organism
medicine 1,2-alpha-L-fucosynthase, derived from an inverting alpha-glycosidase (AfcA) and from a glycosidase with an unusual reaction mechanism, may serve as a promising tool to create biologically active compounds that can be used not only for prebiotics but also for clinical treatments aimed to regulate various cellular processes and infectious diseases Bifidobacterium bifidum

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and mutant derivatives of the catalytic domain of AfcA (Fuc domain) Bifidobacterium bifidum

Protein Variants

Protein Variants Comment Organism
D766G most effectively synthesizes 2'-fucosyllactose Bifidobacterium bifidum
N421G synthesizes 2'-fucosyllactose Bifidobacterium bifidum
N423G synthesizes 2'-fucosyllactose Bifidobacterium bifidum

Organism

Organism UniProt Comment Textmining
Bifidobacterium bifidum
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-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and mutant derivatives of the catalytic domain of AfcA (Fuc domain) Bifidobacterium bifidum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-fucosyllactose + H2O hydrolysis by the Fuc domain of AfcA. N423 and D766 are critical for activating an attacking water molecule and N421 is critical for maintaining the water molecule and the side-chain of E566 (a general acid) in their proper orientations Bifidobacterium bifidum ?
-
 ?
beta-L-fucosyl fluoride + lactose + H2O Hehre resynthesis-hydrolysis is catalyzed by the wild-type Fuc domain of AfcA. Conversion of the inverting alpha-glycosidase to a glycosynthase Bifidobacterium bifidum ?
-
 ?

Synonyms

Synonyms Comment Organism
AfcA
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 Bifidobacterium bifidum