Activating Compound | Comment | Organism | Structure |
---|---|---|---|
maltose | 10 mM, 10% activation | Bacillus cereus | |
NEM | 1 mM, 20% activation | Bacillus cereus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
BaCl2 | 1 mM, 63% inhibition | Bacillus cereus | |
CaCl2 | 1 mM, 60% inhibition | Bacillus cereus | |
CoCl2 | 1 mM, 38% inhibition | Bacillus cereus | |
CuSO4 | 1 mM, 86% inhibition | Bacillus cereus | |
cysteine | 1 mM, 27% inhibition | Bacillus cereus | |
D-mannose | 10 mM, 22% inhibition | Bacillus cereus | |
dithiothreitol | 1 mM, 22% inhibition | Bacillus cereus | |
EDTA | 1 mM, 23% inhibition | Bacillus cereus | |
FeSO4 | 1 mM, complete inhibition | Bacillus cereus | |
glutathione | 1 mM, 21% inhibition | Bacillus cereus | |
Hg2+ | 0.1 mM, complete inhibition | Bacillus cereus | |
L-fucose | 10 mM, 91% inhibition | Bacillus cereus | |
lactose | 10 mM, 27% inhibition | Bacillus cereus | |
NEM | 0.1 mM, 24% inhibition | Bacillus cereus | |
NiSO4 | 1 mM, 59% inhibition | Bacillus cereus | |
p-mercuribenzoate | 1 mM, 23% inhibition | Bacillus cereus | |
ZnCl2 | 1 mM, 43% inhibition | Bacillus cereus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Bacillus cereus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
81000 | - |
2 * 81000, SDS-PAGE | Bacillus cereus |
196000 | - |
gel filtration | Bacillus cereus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Bacillus cereus | inductively produced in culture medium containing porcine gastric mucin, weakly induced by L-fucose and D-arabinose, but not by other sugars including D-glucose | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus cereus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture medium | inductively produced in culture medium containing porcine gastric mucin, weakly induced by L-fucose and D-arabinose, but not by other sugars including glucose | Bacillus cereus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
51.1 | - |
- |
Bacillus cereus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Fucalpha(1-2)Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc + H2O | i.e. lacto-N-fucopentaose I, at 29% of the activity with 2'-fucosyllactose | Bacillus cereus | L-fucose + Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc | - |
? | |
Fucalpha(1-2)Galbeta(1-4)Glc + H2O | i.e. 2'-fucosyllactose | Bacillus cereus | L-fucose + Galbeta(1-4)Glc | - |
? | |
Galbeta(1-4)(Fucalpha(1-3))Glc + H2O | i.e. 3-fucosyllactose, at 0.85% of the activity with 2'-fucosyllactose | Bacillus cereus | ? | - |
? | |
additional information | inductively produced in culture medium containing porcine gastric mucin, weakly induced by L-fucose and D-arabinose, but not by other sugars including D-glucose | Bacillus cereus | ? | - |
? | |
porcine gastric mucin + H2O | at 33% of the activity with 2'-fucosyllactose | Bacillus cereus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 81000, SDS-PAGE | Bacillus cereus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Bacillus cereus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 60 | 20°C: about 45% of maximal activity, 60°C: about 45% of maximal activity | Bacillus cereus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
pH 7.0, 10 min, stable up to | Bacillus cereus |
60 | - |
pH 7.0, 10 min, about 80% loss of activity | Bacillus cereus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Bacillus cereus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | pH 5.0: about 60% of maximal activity, pH 8.0: about 35% of maximal activity | Bacillus cereus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | 37°C, 6 h, stable | Bacillus cereus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bacillus cereus | isoelectric focusing | - |
3 |