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Literature summary for 3.2.1.63 extracted from

  • Miura, T.; Okamoto, K.; Yanase, H.
    Purification and characterization of extracellular 1,2-alpha-L-fucosidase from Bacillus cereus (2005), J. Biosci. Bioeng., 99, 629-635.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
maltose 10 mM, 10% activation Bacillus cereus
NEM 1 mM, 20% activation Bacillus cereus

Inhibitors

Inhibitors Comment Organism Structure
BaCl2 1 mM, 63% inhibition Bacillus cereus
CaCl2 1 mM, 60% inhibition Bacillus cereus
CoCl2 1 mM, 38% inhibition Bacillus cereus
CuSO4 1 mM, 86% inhibition Bacillus cereus
cysteine 1 mM, 27% inhibition Bacillus cereus
D-mannose 10 mM, 22% inhibition Bacillus cereus
dithiothreitol 1 mM, 22% inhibition Bacillus cereus
EDTA 1 mM, 23% inhibition Bacillus cereus
FeSO4 1 mM, complete inhibition Bacillus cereus
glutathione 1 mM, 21% inhibition Bacillus cereus
Hg2+ 0.1 mM, complete inhibition Bacillus cereus
L-fucose 10 mM, 91% inhibition Bacillus cereus
lactose 10 mM, 27% inhibition Bacillus cereus
NEM 0.1 mM, 24% inhibition Bacillus cereus
NiSO4 1 mM, 59% inhibition Bacillus cereus
p-mercuribenzoate 1 mM, 23% inhibition Bacillus cereus
ZnCl2 1 mM, 43% inhibition Bacillus cereus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Bacillus cereus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
81000
-
2 * 81000, SDS-PAGE Bacillus cereus
196000
-
gel filtration Bacillus cereus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Bacillus cereus inductively produced in culture medium containing porcine gastric mucin, weakly induced by L-fucose and D-arabinose, but not by other sugars including D-glucose ?
-
?

Organism

Organism UniProt Comment Textmining
Bacillus cereus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus cereus

Source Tissue

Source Tissue Comment Organism Textmining
culture medium inductively produced in culture medium containing porcine gastric mucin, weakly induced by L-fucose and D-arabinose, but not by other sugars including glucose Bacillus cereus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
51.1
-
-
Bacillus cereus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Fucalpha(1-2)Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc + H2O i.e. lacto-N-fucopentaose I, at 29% of the activity with 2'-fucosyllactose Bacillus cereus L-fucose + Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc
-
?
Fucalpha(1-2)Galbeta(1-4)Glc + H2O i.e. 2'-fucosyllactose Bacillus cereus L-fucose + Galbeta(1-4)Glc
-
?
Galbeta(1-4)(Fucalpha(1-3))Glc + H2O i.e. 3-fucosyllactose, at 0.85% of the activity with 2'-fucosyllactose Bacillus cereus ?
-
?
additional information inductively produced in culture medium containing porcine gastric mucin, weakly induced by L-fucose and D-arabinose, but not by other sugars including D-glucose Bacillus cereus ?
-
?
porcine gastric mucin + H2O at 33% of the activity with 2'-fucosyllactose Bacillus cereus ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 81000, SDS-PAGE Bacillus cereus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Bacillus cereus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 60 20°C: about 45% of maximal activity, 60°C: about 45% of maximal activity Bacillus cereus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
pH 7.0, 10 min, stable up to Bacillus cereus
60
-
pH 7.0, 10 min, about 80% loss of activity Bacillus cereus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Bacillus cereus

pH Range

pH Minimum pH Maximum Comment Organism
5 8 pH 5.0: about 60% of maximal activity, pH 8.0: about 35% of maximal activity Bacillus cereus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9 37°C, 6 h, stable Bacillus cereus

pI Value

Organism Comment pI Value Maximum pI Value
Bacillus cereus isoelectric focusing
-
3