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Literature summary for 3.2.1.63 extracted from

  • Katayama, T.; Sakuma, A.; Kimura, T.; Makimura, Y.; Hiratake, J.; Sakata, K.; Yamanoi, T.; Kumagai, H.; Yamamoto, K.
    Molecular cloning and characterization of Bifidobacterium bifidum 1,2-alpha-L-fucosidase (AfcA), a novel inverting glycosidase (glycoside hydrolase family 95) (2004), J. Bacteriol., 186, 4885-4893.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expression of 1,2-alpha-L-fucosidase in Escherichia coli, expression of the Fuc domain (responsible for fucosidase activity) as a hexahistidine-tagged protein in Escherichia coli Bifidobacterium bifidum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.53
-
 2'-fucosyllactose Fuc domain Bifidobacterium bifidum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
205000
-
 x * 205000, calculated from sequence Bifidobacterium bifidum

Organism

Organism UniProt Comment Textmining
Bifidobacterium bifidum Q6JV24
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Bifidobacterium bifidum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-L-fucosyllactose + H2O reaction with recombinant Fuc domain Bifidobacterium bifidum L-fucose + lactose
-
 ?
blood group substance H + H2O 74% of the activity with 2'-fucosyllactose, reaction with recombinant Fuc domain Bifidobacterium bifidum ?
-
 ?
lacto-N-fucopentaose I + H2O 61% of the activity with 2'-fucosyllactose, reaction with recombinant Fuc domain Bifidobacterium bifidum ?
-
 ?
additional information the recombinant Fuc domain shows no activity with 3-fucosyllactose, lacto-N-fucopentaose II, lacto-N-fucopentaose V and blood group substance B Bifidobacterium bifidum ?
-
 ?

Subunits

Subunits Comment Organism
? x * 205000, calculated from sequence Bifidobacterium bifidum

Synonyms

Synonyms Comment Organism
AfcA
-
 Bifidobacterium bifidum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
 30 min, Fuc domain is stable below Bifidobacterium bifidum

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 7.5 Fuc domain is stable for 12 h Bifidobacterium bifidum