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Literature summary for 3.2.1.62 extracted from

  • Ben Bdira, F.; Jiang, J.; Kallemeijn, W.; de Haan, A.; Florea, B.I.; Bleijlevens, B.; Boot, R.; Overkleeft, H.S.; Aerts, J.M.; Ubbink, M.
    Hydrophobic interactions contribute to conformational stabilization of endoglycoceramidase II by mechanism-based probes (2016), Biochemistry, 55, 4823-4835 .
    View publication on PubMed
Search for more literature for 3.2.1.62

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) pLysS cells Rhodococcus sp. M777

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 1% (v/v) Triton X-100, 10% (v/v) glycerol, and 25% (w/v) PEG Rhodococcus sp. M777

Inhibitors

Inhibitors Comment Organism Structure
nonyl-deoxynojirimycin
-
 Rhodococcus sp. M777

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GM3 ganglioside + H2O Rhodococcus sp. M777
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rhodococcus sp. M777 O33853
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity column chromatography Rhodococcus sp. M777

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl beta-D-lactopyranoside + H2O
-
 Rhodococcus sp. M777 4-methylumbelliferol + beta-D-lactopyranose
-
 ?
GM3 ganglioside + H2O
-
 Rhodococcus sp. M777 ?
-
 ?

Subunits

Subunits Comment Organism
monomer or dimer x * 34000 + x * 25000, SDS-PAGE Rhodococcus sp. M777

Synonyms

Synonyms Comment Organism
EGCII
-
 Rhodococcus sp. M777
endoglycoceramidase II
-
 Rhodococcus sp. M777

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Rhodococcus sp. M777

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 after 1 h at 25°C in 150mM McIlvaine's buffer (pH 5.5), the enzyme remains stable with no changes in its secondary structure and its active state Rhodococcus sp. M777
42
-
 melting temperature Rhodococcus sp. M777

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 in 150 mM McIlvaine's buffer Rhodococcus sp. M777