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Literature summary for 3.2.1.62 extracted from

  • Hayashi, Y.; Okino, N.; Kakuta, Y.; Shikanai, T.; Tani, M.; Narimatsu, H.; Ito, M.
    Klotho-related protein is a novel cytosolic neutral beta-glycosylceramidase (2007), J. Biol. Chem., 282, 30889-30900.
    View publication on PubMed
Search for more literature for 3.2.1.62

Activating Compound

Activating Compound Comment Organism Structure
sodium cholate increases the solubility of the substrates Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression in Chinese hamster ovary-derived cells expressing polyoma LT antigen Mus musculus
expression in Chinese hamster ovary-derived cells expressing polyoma LT antigen, in HEK293 cells cotransfected with in KlrP siRNA, overexpression in Escherichia coli Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
of the recombinant protein with the hanging drops by vapor diffusion method Homo sapiens

Protein Variants

Protein Variants Comment Organism
E165D reduced activity Homo sapiens
E165Q almost no activity Homo sapiens
E373D reduced activity Homo sapiens
E373Q almost no activity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information conduritol B epoxide only slightly inhibitory Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.04
-
 C6-4-nitrobenzo-2-oxa-1,3-diazole-galctosylceramide
-
 Homo sapiens
4.64
-
 (6-[N-(7-nitrobenz-2-oxa-1,3 diazol-4-yl)amino]hexanoyl)-beta-D-glucosylceramide
-
 Homo sapiens
9.24
-
 C18-galactosylceramide
-
 Homo sapiens
13.67
-
 C18-glucosylceramide
-
 Homo sapiens
49.28
-
 4-methylumbelliferyl-beta-D-glucopyranoside
-
 Homo sapiens
144.5
-
 4-methylumbelliferyl-beta-D-galactopyranoside
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Mus musculus 5829
-
cytosol
-
 Homo sapiens 5829
-
cytosol
-
 Rattus norvegicus 5829
-
cytosol
-
 Danio rerio 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
 SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Danio rerio
-
-
-
Homo sapiens
-
-
-
Mus musculus
-
-
-
Rattus norvegicus
-
-
-
Tetraodontidae
-
 puffer fish, no activity in Mycetozoa, slime mold
-

Purification (Commentary)

Purification (Comment) Organism
of the recombinant protein by a hi-trap chelating HP column and superdex 200 column chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Rattus norvegicus
-
embryo
-
 Danio rerio
-
fibroblast
-
 Homo sapiens
-
liver
-
 Mus musculus
-
liver
-
 Tetraodontidae
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00016
-
 activity in CHOP cell lysates transformed with cDNA encoding KLPH, with conduritol B epoxide, C6-4-nitrobenzo-2-oxa-1,3-diazole-galactosylceramide as substrate Mus musculus
0.00025
-
 activity in CHOP cell lysates transformed with cDNA encoding KLPH, without conduritol B epoxide, C6-4-nitrobenzo-2-oxa-1,3-diazole-galactosylceramide as substrate Mus musculus
0.0005
-
 activity in CHOP cell lysates transformed with cDNA encoding KLPH, with conduritol B epoxide, C6-4-nitrobenzo-2-oxa-1,3-diazole-glucosylceramide as substrate Mus musculus
0.0005
-
 in membrane fraction with conduritol B epoxide, C6-4-nitrobenzo-2-oxa-1,3-diazole-glucosylceramide as substrate Rattus norvegicus
0.00133
-
 in cytosolic fraction with conduritol B epoxide, C6-4-nitrobenzo-2-oxa-1,3-diazole-glucosylceramide as substrate Rattus norvegicus
0.001875
-
 activity in CHOP cell lysates transformed with cDNA encoding KlrP, with conduritol B epoxide, C6-4-nitrobenzo-2-oxa-1,3-diazole-glucosylceramide as substrate Homo sapiens
0.0025
-
 activity in CHOP cell lysates transformed with cDNA encoding KLPH, without conduritol B epoxide, C6-4-nitrobenzo-2-oxa-1,3-diazole-glucosylceramide as substrate Mus musculus
0.00375
-
 activity in CHOP cell lysates transformed with cDNA encoding KlrP, without conduritol B epoxide, C6-4-nitrobenzo-2-oxa-1,3-diazole-glucosylceramide as substrate Homo sapiens
0.0052
-
 activity in CHOP cell lysates transformed with cDNA encoding KlrP, with conduritol B epoxide, C6-4-nitrobenzo-2-oxa-1,3-diazole-galactosylceramide as substrate Homo sapiens
0.0066
-
 activity in CHOP cell lysates transformed with cDNA encoding KlrP, without conduritol B epoxide, C6-4-nitrobenzo-2-oxa-1,3-diazole-galactosylceramide as substrate Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-beta-D-galactopyranoside + H2O
-
 Homo sapiens 4-methylumbelliferone + D-galactose
-
 ?
4-methylumbelliferyl-beta-D-glucopyranoside + H2O
-
 Homo sapiens 4-methylumbelliferone + D-glucose
-
 ?
C18-galactosylceramide + H2O
-
 Homo sapiens C18-ceramide + D-galactose
-
 ?
C18-glucosylceramide + H2O
-
 Homo sapiens C18-ceramide + D-glucose
-
 ?
C6-4-nitrobenzo-2-oxa-1,3-diazole-galactosylceramide + H2O
-
 Mus musculus C6-4-nitrobenzo-2-oxa-1,3-diazole-ceramide + D-galactose
-
 ?
C6-4-nitrobenzo-2-oxa-1,3-diazole-galactosylceramide + H2O
-
 Homo sapiens C6-4-nitrobenzo-2-oxa-1,3-diazole-ceramide + D-galactose
-
 ?
C6-4-nitrobenzo-2-oxa-1,3-diazole-glucosylceramide + H2O C6-NBD-glucosylceramide Mus musculus C6-4-nitrobenzo-2-oxa-1,3-diazole-ceramide + D-glucose
-
 ?
C6-4-nitrobenzo-2-oxa-1,3-diazole-glucosylceramide + H2O C6-NBD-glucosylceramide Homo sapiens C6-4-nitrobenzo-2-oxa-1,3-diazole-ceramide + D-glucose
-
 ?
C6-4-nitrobenzo-2-oxa-1,3-diazole-glucosylceramide + H2O C6-NBD-glucosylceramide Rattus norvegicus C6-4-nitrobenzo-2-oxa-1,3-diazole-ceramide + D-glucose
-
 ?
C6-4-nitrobenzo-2-oxa-1,3-diazole-glucosylceramide + H2O C6-NBD-glucosylceramide Danio rerio C6-4-nitrobenzo-2-oxa-1,3-diazole-ceramide + D-glucose
-
 ?
C6-4-nitrobenzo-2-oxa-1,3-diazole-glucosylceramide + H2O C6-NBD-glucosylceramide Tetraodontidae C6-4-nitrobenzo-2-oxa-1,3-diazole-ceramide + D-glucose
-
 ?
galactosylceramide + H2O
-
 Homo sapiens ceramide + D-galactose
-
 ?
galactosylsphingosine + H2O
-
 Homo sapiens sphingosine + D-galactose
-
 ?
glucosylceramide + H2O no activity with alpha-galactosylceramide, lactosylceramide, ganglioside M1a and sulfatides Homo sapiens ceramide + D-glucose
-
 ?
glucosylsphingosine + H2O
-
 Homo sapiens beta-D-glucose + N-acylsphingosine
-
 ?
glycosyl-N-acylsphingosine + H2O
-
 Mus musculus N-acylsphingosine + a sugar
-
 ?
glycosyl-N-acylsphingosine + H2O
-
 Danio rerio N-acylsphingosine + a sugar
-
 ?
glycosyl-N-acylsphingosine + H2O
-
 Tetraodontidae N-acylsphingosine + a sugar
-
 ?

Synonyms

Synonyms Comment Organism
Gcase
-
 Homo sapiens
Gcase
-
 Rattus norvegicus
Gcase
-
 Danio rerio
Gcase
-
 Tetraodontidae
glycosyl-N-acylsphingosine glycohydrolase
-
 Mus musculus
glycosyl-N-acylsphingosine glycohydrolase
-
 Homo sapiens
glycosyl-N-acylsphingosine glycohydrolase
-
 Rattus norvegicus
glycosyl-N-acylsphingosine glycohydrolase
-
 Danio rerio
glycosyl-N-acylsphingosine glycohydrolase
-
 Tetraodontidae
glycosylceramidase
-
 Mus musculus
glycosylceramidase
-
 Homo sapiens
glycosylceramidase
-
 Rattus norvegicus
glycosylceramidase
-
 Danio rerio
glycosylceramidase
-
 Tetraodontidae
KLotho lactase phlorizin hydrolase KLPH Mus musculus
Klotho-related protein KlrP, novel cytosolic neutral glycosylceramidase Homo sapiens
Klotho-related protein KlrP, novel cytosolic neutral glycosylceramidase Rattus norvegicus
Klotho-related protein KlrP, novel cytosolic neutral glycosylceramidase Danio rerio
Klotho-related protein KlrP, novel cytosolic neutral glycosylceramidase Tetraodontidae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7 C6-4-nitrobenzo-2-oxa-1,3-diazole-glucosylceramide as substrate Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
5 8
-
 Homo sapiens