Application | Comment | Organism |
---|---|---|
medicine | rotavirus infection impairs LPH activity in intestinal cells, the decrease in enzyme activity is not Ca2+- and cAMP-dependent, the LPH biosynthesis, stability, and expression is not modified, the impairment of lactase enzymatic activity during rotavirus infection results from an inhibitory action of the secreted non-structural rotavirus protein NSP4 | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NSP4 | secreted non-structural rotavirus protein | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
28.9 | - |
lactose | in infected cells, in brush border-membrane enriched fraction | Homo sapiens | |
30 | - |
lactose | in non infected cells, in brush border-membrane enriched fraction | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
160000 | - |
SDS-PAGE, cleaved mature form | Homo sapiens |
220000 | - |
SDS-PAGE, high mannose form | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by magnesium precipitation method | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
Caco-2 cell | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00164 | - |
in non infected cells, cell homogenates | Homo sapiens |
0.00167 | - |
in cells infected with rhesus monkey rotavirus, cell homogenates | Homo sapiens |
0.00219 | - |
in cells infected with rhesus monkey rotavirus, in brush border-membrane enriched fraction | Homo sapiens |
0.0028 | - |
in cells infected with rhesus monkey rotavirus and NSP4 neutralizing antibodies, in brush border-membrane enriched fraction | Homo sapiens |
0.00334 | - |
in non infected cells, in brush border-membrane enriched fraction | Homo sapiens |
0.00359 | - |
in cells infected with inactivated rhesus monkey rotavirus, in brush border-membrane enriched fraction | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycosyl-N-acylsphingosine + H2O | - |
Homo sapiens | N-acylsphingosine + a sugar | - |
? | |
lactose + H2O | - |
Homo sapiens | D-glucose + D-galactose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glycosyl-N-acylsphingosine glycohydrolase | - |
Homo sapiens |
glycosylceramidase | - |
Homo sapiens |
lactase-phlorizin hydrolase | brush border membrane (BBM)-associated enzyme in intestinal cells | Homo sapiens |
LPH | - |
Homo sapiens |