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Literature summary for 3.2.1.62 extracted from
- Enzymatic hydrolysis of pyridoxine-5'-beta-D-glucoside is catalyzed by intestinal lactase-phlorizin hydrolase (2002), J. Biol. Chem., 277, 26858-26864.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cellobiose | - |
Rattus norvegicus |  |
| D-glucose | - |
Rattus norvegicus | |
| lactose | competitive to pyridoxine-5'-beta-D-glucoside | Rattus norvegicus | |
| additional information | not inhibitory: pyridoxine, 2,4-dinitrophenyl-2-fluoro-2-deoxy-beta-D-glucopyranoside | Rattus norvegicus | |
| pyridoxine-5'-beta-D-glucoside | substrate inhibition above 3 mM | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1 | - |
pyridoxine-5'-beta-D-glucoside | pH 6.0, 37°C | Rattus norvegicus | |
| 16 | - |
lactose | pH 6.0, 37°C | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Rattus norvegicus | 16020 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | precursor form of 200000-220000 Da | Rattus norvegicus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| small intestine | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lactose + H2O | - |
Rattus norvegicus | ? | - |
? | |
| pyridoxine-5'-beta-D-glucoside + H2O | - |
Rattus norvegicus | pyridoxine + D-glucose | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 135000-140000, SDS-PAGE, mature protein | Rattus norvegicus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1 | - |
pyridoxine-5'-beta-D-glucoside | pH 6.0, 37°C | Rattus norvegicus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 56 | - |
lactose | pH 6.0, 37°C | Rattus norvegicus | |
| 64 | - |
D-glucose | pH 6.0, 37°C | Rattus norvegicus | |
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Release 2023.1 (January 2023)
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