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Literature summary for 3.2.1.62 extracted from

  • Ramaswamy, S.; Radhakrishnan, A.N.
    Lactase-phlorizin hydrolase complex from monkey small intestine. Purification, properties and evidence for two catalytic sites (1975), Biochim. Biophys. Acta, 403, 446-455.
    View publication on PubMed
Search for more literature for 3.2.1.62

Inhibitors

Inhibitors Comment Organism Structure
cerebroside from brain, competitive inhibition of lactase activity, Ki: 1.11 mM Macaca radiata
phlorizin competitive inhibition of lactase activity, Ki: 0.18 mM Macaca radiata
sodium cholate competitive inhibition of lactase activity, in the presence of bovine serum albumin Macaca radiata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
 phlorizin
-
 Macaca radiata
21.8
-
 4-O-beta-D-galactopyranosyl-D-glucopyranoside
-
 Macaca radiata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O Macaca radiata low activity 2-hydroxymethylphenol + beta-D-glucose
-
 ?
(2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O Macaca radiata salicin 2-hydroxymethylphenol + beta-D-glucose
-
 ?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O Macaca radiata best substrate D-galactose + beta-D-glucose
-
 ?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O Macaca radiata two enzymatic activities, one for lactose- and one for phlorizin-and lactose-hydrolysis D-galactose + beta-D-glucose
-
 ?
4-O-beta-D-glucopyranosyl-D-glucose + H2O Macaca radiata cellobiose beta-D-glucose + D-glucose
-
 ?
phlorizin + H2O Macaca radiata low activity phloretin + beta-D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Macaca radiata
-
 monkey
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography techniques Macaca radiata

Source Tissue

Source Tissue Comment Organism Textmining
intestine small intestine Macaca radiata
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.45
-
 phlorizin activity Macaca radiata
16.1
-
 lactase activity Macaca radiata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O low activity Macaca radiata 2-hydroxymethylphenol + beta-D-glucose
-
 ?
(2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O salicin Macaca radiata 2-hydroxymethylphenol + beta-D-glucose
-
 ?
2-nitrophenyl beta-D-glucopyranoside + H2O
-
 Macaca radiata 2-nitrophenol + beta-D-glucose
-
 ?
4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Macaca radiata 4-nitrophenol + beta-D-galactose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Macaca radiata 4-nitrophenol + beta-D-glucose
-
 ?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O best substrate Macaca radiata D-galactose + beta-D-glucose
-
 ?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O two enzymatic activites, one for lactose- and one for phlorizin-and lactose-hydrolysis Macaca radiata D-galactose + beta-D-glucose
-
 ?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O two enzymatic activities, one for lactose- and one for phlorizin-and lactose-hydrolysis Macaca radiata D-galactose + beta-D-glucose
-
 ?
4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Macaca radiata beta-D-glucose + D-glucose
-
 ?
beta-methylglucoside + H2O
-
 Macaca radiata methanol + beta-D-glucose
-
 ?
N-palmitoyldihydroglucosylcerebroside + H2O 1.5% of the activity towards lactose Macaca radiata beta-D-glucose + N-palmitoyldihydrocerebroside
-
 ?
phlorizin + H2O low activity Macaca radiata phloretin + beta-D-glucose
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 complete inactivation Macaca radiata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6
-
 lactase activity, phlorizin activity in the acidic pH range Macaca radiata

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.11
-
 cerebroside from brain, competitive inhibition of lactase activity Macaca radiata