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Literature summary for 3.2.1.62 extracted from

  • Cousineau, J.; Green, J.R.
    Isolation and characterization of the proximal and distal forms of lactase-phlorizin hydrolase from the small intestine of the suckling rat (1980), Biochim. Biophys. Acta, 615, 147-157.
    View publication on PubMed
Search for more literature for 3.2.1.62

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.73
-
 N-palmitoyldihydrocerebroside from proximal intestine isolated phlorizin hydrolase activity Rattus sp.
1.12
-
 N-palmitoyldihydrocerebroside from distal intestine isolated phlorizin hydrolase activity Rattus sp.
14.8
-
 4-O-beta-D-galactopyranosyl-D-glucopyranoside from proximal intestine isolated lactase Rattus sp.
19.1
-
 4-O-beta-D-galactopyranosyl-D-glucopyranoside from distal intestine isolated lactase Rattus sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
brush border
-
 Rattus sp. 5903
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O Rattus sp.
-
 D-galactose + beta-D-glucose
-
 ?
N-palmitoyldihydroglucosylceramide + H2O Rattus sp.
-
 beta-D-glucose + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
two isoenzymes from proximal and distal intestine, to homogeneity, chromatography techniques Rattus sp.

Source Tissue

Source Tissue Comment Organism Textmining
intestine
-
 Rattus sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
22
-
-
 Rattus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
-
 Rattus sp. D-galactose + beta-D-glucose
-
 ?
N-palmitoyldihydroglucosylceramide + H2O
-
 Rattus sp. beta-D-glucose + ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 stable for 60 min Rattus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 both isoforms Rattus sp.