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Literature summary for 3.2.1.61 extracted from

  • Okazaki, K.; Abe, T.; Saruwatari, K.; Kato, F.; Maruyama, K.; Tagawa, K.
    Purification and properties of mycodextranase from Streptomyces sp. J-13-3 (1992), Biosci. Biotechnol. Biochem., 56, 1401-1405.
    View publication on PubMed
Search for more literature for 3.2.1.61

Inhibitors

Inhibitors Comment Organism Structure
Hg+
-
 Penicillium melinii
Hg+
-
 Streptomyces sp.
Hg2+
-
 Streptomyces sp.
p-chloromercuribenzoic acid
-
 Streptomyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
 1 * 68000, SDS-PAGE Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Chloridium sp.
-
-
-
Cladosporium sp.
-
-
-
Penicillium melinii
-
-
-
Streptomyces griseus
-
 IFO 3355
-
Streptomyces sp.
-
 J-13-3
-
Streptomyces sp. J-13-3
-
 J-13-3
-
Trichoderma sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces sp.

Reaction

Reaction Comment Organism Reaction ID
nigerohexaose + H2O = nigerose + alpha-D-glucopyranosyl-1,3-alpha-D-glucopyranosyl-1,4-alpha-D-glucopyranosyl-1,3-D-glucose enzyme hydrolyzes nigeran tetrasaccharide by an endo-type of action Streptomyces sp.
a
nigerohexaose + H2O = nigerose + alpha-D-glucopyranosyl-1,3-alpha-D-glucopyranosyl-1,4-alpha-D-glucopyranosyl-1,3-D-glucose endo-multichain mechanism Penicillium melinii
a
nigerohexaose + H2O = nigerose + alpha-D-glucopyranosyl-1,3-alpha-D-glucopyranosyl-1,4-alpha-D-glucopyranosyl-1,3-D-glucose endo-multichain mechanism Chloridium sp.
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.53
-
-
 Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nigeran + H2O
-
 Streptomyces griseus nigerose + alpha-D-glucopyranosyl-1,3-alpha-D-glucopyranosyl-1,4-alpha-D-glucopyranosyl-1,3-D-glucose
-
 ?
nigeran + H2O
-
 Streptomyces sp. nigerose + alpha-D-glucopyranosyl-1,3-alpha-D-glucopyranosyl-1,4-alpha-D-glucopyranosyl-1,3-D-glucose
-
 ?
nigeran + H2O
-
 Cladosporium sp. nigerose + alpha-D-glucopyranosyl-1,3-alpha-D-glucopyranosyl-1,4-alpha-D-glucopyranosyl-1,3-D-glucose
-
 ?
nigeran + H2O
-
 Penicillium melinii nigerose + alpha-D-glucopyranosyl-1,3-alpha-D-glucopyranosyl-1,4-alpha-D-glucopyranosyl-1,3-D-glucose
-
 ?
nigeran + H2O
-
 Chloridium sp. nigerose + alpha-D-glucopyranosyl-1,3-alpha-D-glucopyranosyl-1,4-alpha-D-glucopyranosyl-1,3-D-glucose
-
 ?
nigeran + H2O
-
 Trichoderma sp. nigerose + alpha-D-glucopyranosyl-1,3-alpha-D-glucopyranosyl-1,4-alpha-D-glucopyranosyl-1,3-D-glucose
-
 ?
nigeran + H2O
-
 Streptomyces sp. J-13-3 nigerose + alpha-D-glucopyranosyl-1,3-alpha-D-glucopyranosyl-1,4-alpha-D-glucopyranosyl-1,3-D-glucose
-
 ?

Subunits

Subunits Comment Organism
monomer
-
 Penicillium melinii
monomer 1 * 68000, SDS-PAGE Streptomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Streptomyces sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 10 min, 60% of maximal activity Streptomyces sp.
70
-
 10 min, 22% of maximal activity Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Streptomyces sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8 stable between Streptomyces sp.