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Literature summary for 3.2.1.6 extracted from

  • Kobayashi, T.; Uchimura, K.; Kubota, T.; Nunoura, T.; Deguchi, S.
    Biochemical and genetic characterization of beta-1,3 glucanase from a deep subseafloor Laceyella putida (2016), Appl. Microbiol. Biotechnol., 100, 203-214.
    View publication on PubMed
Search for more literature for 3.2.1.6

Inhibitors

Inhibitors Comment Organism Structure
2-Hydroxy-5-nitrobenzylbromide 5 mM, 47% inhibition Laceyella putida
Hg2+ 1 mM, 39% inhibition Laceyella putida
iodoacetic acid 5 mM, 34% inhibition Laceyella putida
Pd2+ 1 mM, 23% inhibition Laceyella putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.21
-
 laminaritetraose pH 4.0, 50°C Laceyella putida
2.39
-
 Laminaripentaose pH 4.0, 50°C Laceyella putida
2.73
-
 Laminarihexaose pH 4.0, 50°C Laceyella putida

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Laceyella putida
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
-
 Laceyella putida

Organism

Organism UniProt Comment Textmining
Laceyella putida A0A0P0UPC6
-
-
Laceyella putida JAM FM3001 A0A0P0UPC6
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Laceyella putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
curdlan + H2O
-
 Laceyella putida laminaribiose + laminaritriose + ? main products ?
laminarihexaose + H2O
-
 Laceyella putida D-glucose + laminaribiose + laminaritriose
-
 ?
laminarin + H2O 48% of the activity with curdlan Laceyella putida ?
-
 ?
laminarin + H2O 48% of the activity with curdlan Laceyella putida JAM FM3001 ?
-
 ?
laminaripentaose + H2O
-
 Laceyella putida D-glucose + laminaribiose + laminaritriose
-
 ?
laminaritetraose + H2O
-
 Laceyella putida D-glucose + laminaribiose + laminaritriose
-
 ?
laminaritetraose + H2O
-
 Laceyella putida JAM FM3001 D-glucose + laminaribiose + laminaritriose
-
 ?
laminaritriose + H2O
-
 Laceyella putida D-glucose + laminaribiose
-
 ?
laminaritriose + H2O
-
 Laceyella putida JAM FM3001 D-glucose + laminaribiose
-
 ?
additional information no substrates: xylan, colloidal chitin, carboxymethyl cellulose, polygalacturonic acid, pectin and laminaribiose Laceyella putida ?
-
 ?
additional information no substrates: xylan, colloidal chitin, carboxymethyl cellulose, polygalacturonic acid, pectin and laminaribiose Laceyella putida JAM FM3001 ?
-
 ?
pachyman + H2O 223% of the activity with curdlan Laceyella putida ?
-
 ?
pachyman + H2O 223% of the activity with curdlan Laceyella putida JAM FM3001 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 36000, SDS-PAGE, x * 39836, calculated Laceyella putida

Synonyms

Synonyms Comment Organism
GluA
-
 Laceyella putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
 Laceyella putida

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 enzyme shows relatively low thermostability, which is stabilized by adding laminarin, xylan, colloidal chitin, pectin, and its related polysaccharides Laceyella putida
30 75 30 min, pH 7.5, 20 mM Tris-HCl, 35-45% residual activtiy Laceyella putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
188
-
 laminaritetraose pH 4.0, 50°C Laceyella putida
355
-
 Laminaripentaose pH 4.0, 50°C Laceyella putida
582
-
 Laminarihexaose pH 4.0, 50°C Laceyella putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2
-
-
 Laceyella putida

pH Stability

pH Stability pH Stability Maximum Comment Organism
2.4 8.7 40°C, 30 min, about 50% residual activity Laceyella putida

pI Value

Organism Comment pI Value Maximum pI Value
Laceyella putida calculated
-
 5