Cloned (Comment) | Organism |
---|---|
recombinant expression of wild-type and deletion mutant enzymes in Escherichia coli strain Rosetta-gami B (DE3), secretion of enzyme to the culture medium, recombinant expression as GFP-tagged proteins, overview | Niallia circulans |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of domain deletion enzyme mutants lacking discoidin domains DS1 and DS2, the uncharacterized conserved domain, and/or carbohydrate binding module family 6, AglDELTADS1, AglDELTADS1CB6, AglDELTADS1CB6DS2, and AglDELTADCD-UCD. Only the DS1 domain deletion mutant shows some activity, the other mutants show only residual or no activity, overview | Niallia circulans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km is 5.6 mg/ml for alpha-1,3-glucan with the recombinant wild-type enzyme, pH 6.5, 30°C, kinetics of recombinant wild-type and domain deletion mutants, overview | Niallia circulans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Niallia circulans | - |
- |
- |
Niallia circulans KA-304 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant secreted wild-type and deletion mutant enzymes from Escherichia coli strain Rosetta-gami B (DE3) culture supernatant by dialysis, anion exchange chromatography, ammonium sulfate fractionation and hydrophobic interaction chromatography | Niallia circulans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-1,3-glucan + H2O | prepared from sucrose using Streptococcus mutans ATCC700610 glucosyltransferase I | Niallia circulans | ? | - |
? | |
alpha-1,3-glucan + H2O | prepared from sucrose using Streptococcus mutans ATCC700610 glucosyltransferase I | Niallia circulans KA-304 | ? | - |
? | |
fungal cell walls + H2O | - |
Niallia circulans | ? | - |
? | |
fungal cell walls + H2O | - |
Niallia circulans KA-304 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme structure includes an N-terminal discoidin domain (DS1), a carbohydrate binding module family 6 (CB6), threonine and proline repeats (TPs), a second discoidin domain (DS2), an uncharacterized conserved domain (UCD), and a C-terminal catalytic domain | Niallia circulans |
Synonyms | Comment | Organism |
---|---|---|
Agl-KA | - |
Niallia circulans |
alpha-1,3-glucanase | - |
Niallia circulans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Niallia circulans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the amount of reducing sugar in the supernatant of the enzyme assay is determined | Niallia circulans | |
91.3 | - |
alpha-1,3-glucan | recombinant wild-type enzyme, pH 6.5, 30°C | Niallia circulans | |
106.6 | - |
alpha-1,3-glucan | recombinant mutant AglDELTADS1, pH 6.5, 30°C | Niallia circulans | |
130.7 | - |
alpha-1,3-glucan | recombinant mutant AglDELTADS1CB6, pH 6.5, 30°C | Niallia circulans | |
134.4 | - |
alpha-1,3-glucan | recombinant mutant AglDELTADS1CB6DS2, pH 6.5, 30°C | Niallia circulans | |
158.7 | - |
alpha-1,3-glucan | recombinant mutant AglDELTADCD-UCD, pH 6.5, 30°C | Niallia circulans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Niallia circulans |
General Information | Comment | Organism |
---|---|---|
malfunction | domain deletion enzyme mutants lacking discoidin domains DS1 and DS2 and carbohydrate binding module family 6 domain exhibit lower alpha-1,3-glucan-hydrolyzing and substrate-binding activities than the wild-type enzyme. A dramatic decrease in protoplast formation in the Schizophyllum commune mycelium is observed given only a domain DS1 deletion | Niallia circulans |
additional information | discoidin domains DS1 and DS2 and carbohydrate binding module family 6 domain are involved in alpha-1,3-glucan and fungal cell wall substrate binding, while the uncharacterized conserved domain is not, overview | Niallia circulans |
physiological function | the enzyme is involved in fungal cell wall degradation | Niallia circulans |