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Literature summary for 3.2.1.59 extracted from

  • Dekker, N.; van Rijssel, J.; Distel, B.; Hochstenbach, F.
    Role of the alpha-glucanase Agn2p in ascus-wall endolysis following sporulation in fission yeast (2007), Yeast, 24, 279-288.
    View publication on PubMed

Application

Application Comment Organism
additional information Agn2p participates in the endolysis of the ascus wall by hydrolysing its (1,3)-alpha-glucan, thereby assisting in the release of ascospores Schizosaccharomyces pombe

Cloned(Commentary)

Cloned (Comment) Organism
tagged Agn2p at its carboxyl terminus with six histidines, expression in Escherichia coli Schizosaccharomyces pombe

Protein Variants

Protein Variants Comment Organism
additional information asci deleted for agn2 are defective in ascospore dispersal, but exogenous addition of purified Agn2p liberates the ascospores from asci deleted for agn2 Schizosaccharomyces pombe

Inhibitors

Inhibitors Comment Organism Structure
additional information Agn2p not inhibited by 1-deoxynojirimycin, N-methyl-1-deoxynojirimycin, castanospermin, D-glucono-1,5-lactone, or conduritol-beta-epoxide Schizosaccharomyces pombe

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
50000, Agn2p, SDS-PAGE Schizosaccharomyces pombe

Organism

Organism UniProt Comment Textmining
Schizosaccharomyces pombe
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Agn2p purified by immobilized metal ion affinity chromatography followed by anion exchange chromatography Schizosaccharomyces pombe

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-1,3-glucan + H2O Agn2p-his shows high specificity for (1,3)-alpha-glucosidic linkages Schizosaccharomyces pombe ?
-
?
additional information no activity of Agn2p with (1,3)-alpha-mannan, (1,3)-beta-glucan, (1,4)-alpha-glucan or (1,6)-alpha-glucan Schizosaccharomyces pombe ?
-
?

Subunits

Subunits Comment Organism
? 50000, Agn2p, SDS-PAGE Schizosaccharomyces pombe

Synonyms

Synonyms Comment Organism
Agn1p
-
Schizosaccharomyces pombe
Agn2p
-
Schizosaccharomyces pombe
endo-(1,3)-alpha-glucanase
-
Schizosaccharomyces pombe

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
Agn1p shows a pronounced pH optimum at pH 3, whereas Agn2p shows highest activity to (1,3)-alpha-glucan at pH 6 Schizosaccharomyces pombe