Application | Comment | Organism |
---|---|---|
additional information | Agn2p participates in the endolysis of the ascus wall by hydrolysing its (1,3)-alpha-glucan, thereby assisting in the release of ascospores | Schizosaccharomyces pombe |
Cloned (Comment) | Organism |
---|---|
tagged Agn2p at its carboxyl terminus with six histidines, expression in Escherichia coli | Schizosaccharomyces pombe |
Protein Variants | Comment | Organism |
---|---|---|
additional information | asci deleted for agn2 are defective in ascospore dispersal, but exogenous addition of purified Agn2p liberates the ascospores from asci deleted for agn2 | Schizosaccharomyces pombe |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | Agn2p not inhibited by 1-deoxynojirimycin, N-methyl-1-deoxynojirimycin, castanospermin, D-glucono-1,5-lactone, or conduritol-beta-epoxide | Schizosaccharomyces pombe |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
50000, Agn2p, SDS-PAGE | Schizosaccharomyces pombe |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schizosaccharomyces pombe | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Agn2p purified by immobilized metal ion affinity chromatography followed by anion exchange chromatography | Schizosaccharomyces pombe |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-1,3-glucan + H2O | Agn2p-his shows high specificity for (1,3)-alpha-glucosidic linkages | Schizosaccharomyces pombe | ? | - |
? | |
additional information | no activity of Agn2p with (1,3)-alpha-mannan, (1,3)-beta-glucan, (1,4)-alpha-glucan or (1,6)-alpha-glucan | Schizosaccharomyces pombe | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | 50000, Agn2p, SDS-PAGE | Schizosaccharomyces pombe |
Synonyms | Comment | Organism |
---|---|---|
Agn1p | - |
Schizosaccharomyces pombe |
Agn2p | - |
Schizosaccharomyces pombe |
endo-(1,3)-alpha-glucanase | - |
Schizosaccharomyces pombe |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
Agn1p shows a pronounced pH optimum at pH 3, whereas Agn2p shows highest activity to (1,3)-alpha-glucan at pH 6 | Schizosaccharomyces pombe |