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Literature summary for 3.2.1.59 extracted from

  • Takehara, T.; Inoue, M.; Morioka, T.; Yokogawa, K.
    Purification and properties of endo-alpha-1,3-glucanase from a Streptomyces chartreusis strain (1981), J. Bacteriol., 145, 729-735.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
Streptomyces chartreusis
Cu2+
-
Streptomyces chartreusis
Hg2+
-
Streptomyces chartreusis
Merthiolate
-
Streptomyces chartreusis
Mn2+
-
Streptomyces chartreusis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.89
-
Glucan
-
Streptomyces chartreusis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ no effect Streptomyces chartreusis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
SDS-PAGE Streptomyces chartreusis

Organism

Organism UniProt Comment Textmining
Streptomyces chartreusis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces chartreusis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.968
-
-
Streptomyces chartreusis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glucan + H2O glucan produced from sucrose by Streptococcus mutans Streptomyces chartreusis nigerose + glucose
-
?

Subunits

Subunits Comment Organism
oligomer possibly oligomeric or aggregated form, molecular weight larger than 300000, x * 68000, SDS-PAGE, Streptomyces chartreusis Streptomyces chartreusis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Streptomyces chartreusis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
pH 6.0, 10 min, complete inactivation Streptomyces chartreusis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6
-
Streptomyces chartreusis