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Literature summary for 3.2.1.58 extracted from
- Expression and characterization of a novel antifungal Exo-beta-1,3-glucanase from Chaetomium cupreum (2017), Appl. Biochem. Biotechnol., 182, 261-275 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Pichia pastoris | Arcopilus cupreus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 5 mM, 35% residual activity | Arcopilus cupreus |  |
| Co2+ | 5 mM, 70% residual activity | Arcopilus cupreus | |
| Cu2+ | 5 mM, 63% residual activity | Arcopilus cupreus | |
| Hg2+ | 5 mM, 40% residual activity | Arcopilus cupreus | |
| Mn2+ | 5 mM, 43% residual activity | Arcopilus cupreus | |
| additional information | enzyme is not affected by 5 mM K+, Mg2+, Ca2+, or Zn2+ | Arcopilus cupreus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
Laminarin | Km value 2.84 mg/ml, pH 5.0, 45°C | Arcopilus cupreus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | - |
Arcopilus cupreus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arcopilus cupreus | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| barley beta-glucan + H2O | 7.4% of the activity with laminarin | Arcopilus cupreus | D-glucose + ? | - |
? | |
| laminaribiose + H2O | - |
Arcopilus cupreus | 2 D-glucose | - |
? | |
| laminarin + H2O | - |
Arcopilus cupreus | D-glucose + ? | - |
? | |
| laminaritriose + 2 H2O | - |
Arcopilus cupreus | 3 D-glucose | - |
? | |
| additional information | enzyme almost exclusively catalyzes beta-1,3-glycosidic bond cleavage in various polysaccharoses to liberate glucose and acts as exoglucanase with transglycosylation activity. No substrates: avicel, pustulan, dextran, or amylose | Arcopilus cupreus | ? | - |
? | |
| zymosan A + H2O | 45.4% of the activity with laminarin | Arcopilus cupreus | D-glucose + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 65000, SDS-PAGE, x * 53900, calculated | Arcopilus cupreus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Glu17A | - |
Arcopilus cupreus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Arcopilus cupreus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Arcopilus cupreus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Arcopilus cupreus | calculated | - |
4.8 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | enzyme shows potential antifungal activity with a degradative effect on cell walls and inhibitory action against the germination of pathogenic fungus | Arcopilus cupreus |
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