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Literature summary for 3.2.1.58 extracted from
- Novel alpha-L-arabinofuranosidase from Cellulomonas fimi ATCC 484 and its substrate-specificity analysis with the aid of computer (2015), J. Agric. Food Chem., 63, 3725-3733.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| molecular modeling of structure. Residue Glu173 is the acid/base, and Glu292 is the nucleophile | Cellulomonas fimi |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.5 | - |
4-nitrophenyl-alpha-L-arabinofuranoside | pH 6.0, 40°C | Cellulomonas fimi |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55600 | - |
- |
Cellulomonas fimi |
| 66000 | - |
- |
Cellulomonas fimi |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cellulomonas fimi | F4H166 | - |
- |
| Cellulomonas fimi ATCC 484 | F4H166 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 19.4 | - |
substrate 4-nitrophenyl-alpha-L-arabinofuranoside, pH 6.0, 40°C | Cellulomonas fimi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Cellulomonas fimi | 4-nitrophenol + L-arabinofuranose | - |
? | |
| 4-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Cellulomonas fimi ATCC 484 | 4-nitrophenol + L-arabinofuranose | - |
? | |
| linear alpha-1,5-L-arabinan + H2O | - |
Cellulomonas fimi | ? | - |
? | |
| linear alpha-1,5-L-arabinan + H2O | - |
Cellulomonas fimi ATCC 484 | ? | - |
? | |
| additional information | no substrates: beechwood xylan, soluble starch, gum arabic, and larch arabinogalactan, 4-nitrophenyl alpha-D-xylopyranoside and 4-nitrophenyl beta-D-xylopyranoside | Cellulomonas fimi | ? | - |
? | |
| additional information | no substrates: beechwood xylan, soluble starch, gum arabic, and larch arabinogalactan, 4-nitrophenyl alpha-D-xylopyranoside and 4-nitrophenyl beta-D-xylopyranoside | Cellulomonas fimi ATCC 484 | ? | - |
? | |
| sugar beet arabinan + H2O | - |
Cellulomonas fimi | ? | - |
? | |
| sugar beet arabinan + H2O | - |
Cellulomonas fimi ATCC 484 | ? | - |
? | |
| wheat flour arabinoxylan + H2O | - |
Cellulomonas fimi | ? | - |
? | |
| wheat flour arabinoxylan + H2O | - |
Cellulomonas fimi ATCC 484 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 55600, calculated, x * 66000, SDS-PAGE | Cellulomonas fimi |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Celf_3321 | - |
Cellulomonas fimi |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 123.6 | - |
4-nitrophenyl-alpha-L-arabinofuranoside | pH 6.0, 40°C | Cellulomonas fimi | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Cellulomonas fimi |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
58% of maximum activity | Cellulomonas fimi |
| 7 | - |
72% of maximum activity | Cellulomonas fimi |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
4 h, complete loss of activity | Cellulomonas fimi |
| 6 | - |
stable | Cellulomonas fimi |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Cellulomonas fimi | calculated | - |
4.9 |
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