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Literature summary for 3.2.1.58 extracted from

  • Ooi, T.; Sato, H.; Matsumoto, K.; Taguchi, S.
    A unique post-translational processing of an exo-beta-1,3-glucanase of Penicillium sp. KH10 expressed in Aspergillus oryzae (2009), Protein Expr. Purif., 67, 126-131.
    View publication on PubMed
Search for more literature for 3.2.1.58

Cloned(Commentary)

Cloned (Comment) Organism
gene exgP, phylogenetic analysis, subcloning in Escherichia coli strain JM109, heterologous secretory expression in Aspergillus oryzae, the recombinant enzyme is secreted to the culture medium Penicillium sp. KH10

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the enzyme is secreted to the culture medium Penicillium sp. KH10
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
104000
-
 enzyme precursor, gel filtration Penicillium sp. KH10

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
laminarin + H2O Penicillium sp. KH10 high activity in strain KH10 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Penicillium sp. KH10
-
 gene exgP
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the processed enzyme is N-glycosylated Penicillium sp. KH10

Purification (Commentary)

Purification (Comment) Organism
recombinant ExgP from Aspergillus oryzae culture supernatant by anion exchange and hydrophobic interaction chromatography, and gel filtration Penicillium sp. KH10

Source Tissue

Source Tissue Comment Organism Textmining
cell culture growth on laminarin from Laminaria digitata or beta-glucan from the fruiting bodies of Pleurotus cornucopiae Penicillium sp. KH10
-
hypha
-
 Penicillium sp. KH10
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11.7
-
 purified mature enzyme Penicillium sp. KH10

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-1,3-glucan + H2O
-
 Penicillium sp. KH10 ?
-
 ?
laminarin + H2O high activity in strain KH10 Penicillium sp. KH10 ?
-
 ?
laminarin + H2O from Laminaria digitata or Eisenia bicyclis Penicillium sp. KH10 ?
-
 ?
additional information ExgP is active only toward glucans containing beta-1,3-linkages, such as laminarin and laminaran, no or poor activity with curdran, carboxymethyl-curdlan, lentinan, beta-glucan from Hordeum vulgare, pusturan and carboxymethylcellulose Penicillium sp. KH10 ?
-
 ?

Subunits

Subunits Comment Organism
? two fragements of 42 kDa and 47 kDa, probably generated by proteolytic cleavage Penicillium sp. KH10

Synonyms

Synonyms Comment Organism
ExgP
-
 Penicillium sp. KH10
exo-beta-1,3-glucanase
-
 Penicillium sp. KH10
More the enzyme probably belongs to glycosyl hydrolase family 55, GH55 Penicillium sp. KH10

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Penicillium sp. KH10

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70
-
 Penicillium sp. KH10

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Penicillium sp. KH10

pH Range

pH Minimum pH Maximum Comment Organism
3 6.5
-
 Penicillium sp. KH10