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Literature summary for 3.2.1.58 extracted from

  • Peng, Y.; Chi, Z.M.; Wang, X.H.; Li, J.
    Purification and molecular characterization of exo-beta-1,3-glucanases from the marine yeast Williopsis saturnus WC91-2 (2009), Appl. Microbiol. Biotechnol., 85, 85-94.
    View publication on PubMed
Search for more literature for 3.2.1.58

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Cyberlindnera saturnus
Ag+
-
 Cyberlindnera saturnus
Ca2+
-
 Cyberlindnera saturnus
Co2+
-
 Cyberlindnera saturnus
Cu2+
-
 Cyberlindnera saturnus
EDTA
-
 Cyberlindnera saturnus
EGTA
-
 Cyberlindnera saturnus
Fe2+
-
 Cyberlindnera saturnus
Fe3+
-
 Cyberlindnera saturnus
Hg2+
-
 Cyberlindnera saturnus
iodoacetic acid
-
 Cyberlindnera saturnus
K+
-
 Cyberlindnera saturnus
Mg2+
-
 Cyberlindnera saturnus
Mn2+
-
 Cyberlindnera saturnus
Na+
-
 Cyberlindnera saturnus
PMSF
-
 Cyberlindnera saturnus
Zn2+
-
 Cyberlindnera saturnus

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Cyberlindnera saturnus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ activates Cyberlindnera saturnus
Li+ activates Cyberlindnera saturnus
Ni2+ activates Cyberlindnera saturnus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47500
-
 x * 47500, native enzyme, SDS-PAGE Cyberlindnera saturnus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
laminarin + H2O Cyberlindnera saturnus exo-beta-1,3-glucanases hydrolyze laminarin by sequentially cleaving glucose residues from the non-reducing end of polymers or oligomers ?
-
 ?

Organism

Organism UniProt Comment Textmining
Cyberlindnera saturnus C3VMY4 i.e. Hansenula saturnus, a marine yeast, strain WC91-2, gene WsEXG1
-

Purification (Commentary)

Purification (Comment) Organism
soluble native enzyme 115fold from strain WC91-2 to homogeneity by ultrafiltration, gel filtration, and anion exchange chromatography Cyberlindnera saturnus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1966
-
 purified native enzyme Cyberlindnera saturnus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
laminarin + H2O exo-beta-1,3-glucanases hydrolyze laminarin by sequentially cleaving glucose residues from the non-reducing end of polymers or oligomers Cyberlindnera saturnus ?
-
 ?
laminarin + H2O the purified native enzyme can convert laminarin into monosaccharides and disaccharides, but has no killer toxin activity Cyberlindnera saturnus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 47500, native enzyme, SDS-PAGE Cyberlindnera saturnus

Synonyms

Synonyms Comment Organism
exo-beta-1,3-glucanase
-
 Cyberlindnera saturnus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Cyberlindnera saturnus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 60 activity range Cyberlindnera saturnus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
 Cyberlindnera saturnus

pH Range

pH Minimum pH Maximum Comment Organism
3 7 activity range Cyberlindnera saturnus