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Literature summary for 3.2.1.57 extracted from

  • Akeboshi, H.; Tonozuka, T.
    Insights into the reaction mechanism of glycosol hydrolase family 49. Site-directed mutagenesis and substrate preference of isopullulanase (2004), Eur. J. Biochem., 271, 4420-4427.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
D353N enzyme shows no activity with pullulan and panose as substrate Aspergillus niger
D372N enzyme shows no activity with pullulan and panose as substrate Aspergillus niger
D373N enzyme shows no activity with pullulan and panose as substrate Aspergillus niger
E273N 45% of wild-type activity with pullulan as substrate, 74% of wild-type activity with panose as substrate Aspergillus niger
E356Q 38% of wild-type activity with pullulan as substrate, 50% of wild-type activity with panose as substrate Aspergillus niger
W240F 130% of wild-type activity with pullulan as substrate, 160% of wild-type activity with panose as substrate Aspergillus niger
W31F 38% of wild-type activity with pullulan as substrate, 140% of wild-type activity with panose as substrate Aspergillus niger
W32F 90% of wild-type activity with pullulan as substrate, 120% of wild-type activity with panose as substrate Aspergillus niger
W402F 0.4% of wild-type activity with pullulan as substrate, 0.1% of wild-type activity with panose as substrate Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
160
-
panose pH 3.5, 40°C, wild-type enzyme Aspergillus niger
920
-
panose pH 3.5, 40°C, mutant enzyme W402F Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and mutant enzymes Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
panose + H2O
-
Aspergillus niger isomaltose + glucose
-
?
pullulan + H2O
-
Aspergillus niger isopanose + ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.36
-
panose mutant enzyme W402F Aspergillus niger
180
-
panose wild-type enzyme Aspergillus niger