Protein Variants | Comment | Organism |
---|---|---|
D353N | enzyme shows no activity with pullulan and panose as substrate | Aspergillus niger |
D372N | enzyme shows no activity with pullulan and panose as substrate | Aspergillus niger |
D373N | enzyme shows no activity with pullulan and panose as substrate | Aspergillus niger |
E273N | 45% of wild-type activity with pullulan as substrate, 74% of wild-type activity with panose as substrate | Aspergillus niger |
E356Q | 38% of wild-type activity with pullulan as substrate, 50% of wild-type activity with panose as substrate | Aspergillus niger |
W240F | 130% of wild-type activity with pullulan as substrate, 160% of wild-type activity with panose as substrate | Aspergillus niger |
W31F | 38% of wild-type activity with pullulan as substrate, 140% of wild-type activity with panose as substrate | Aspergillus niger |
W32F | 90% of wild-type activity with pullulan as substrate, 120% of wild-type activity with panose as substrate | Aspergillus niger |
W402F | 0.4% of wild-type activity with pullulan as substrate, 0.1% of wild-type activity with panose as substrate | Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
160 | - |
panose | pH 3.5, 40°C, wild-type enzyme | Aspergillus niger | |
920 | - |
panose | pH 3.5, 40°C, mutant enzyme W402F | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and mutant enzymes | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
panose + H2O | - |
Aspergillus niger | isomaltose + glucose | - |
? | |
pullulan + H2O | - |
Aspergillus niger | isopanose + ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.36 | - |
panose | mutant enzyme W402F | Aspergillus niger | |
180 | - |
panose | wild-type enzyme | Aspergillus niger |