Any feedback?
Literature summary for 3.2.1.57 extracted from
- Deglycosylated isopullulanase retains enzymatic activity (2000), J. Appl. Glycosci., 47, 287-292.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| ipuA gene, expression in Aspergillus oryzae M-2-3 | Aspergillus niger |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic parameters for deglycosylated recombinant IPU with a sugar content of 13.8%, 6.8% and 2.1%, respectively, deglycosylation decreases the kinetic parameters, except Km for pullulan | Aspergillus niger | |
| 0.88 | - |
pullulan | 40°C, recombinant IPU | Aspergillus niger |  |
| 39.2 | - |
panose | 40°C, recombinant IPU | Aspergillus niger | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 59000 | - |
x * 59000, deglycosylated recombinant IPU, SDS-PAGE | Aspergillus niger |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
strain ATCC 9642 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | the sugar content of native IPU is 12.4-15.3%, that of recombinant IPU is 33.8%, deglycosylated enzyme by endoglycosidase H with a sugar content of 2.1% retains 65% of its original activity, kinetics of deglycosylated IPU, hybrid- and/or high-mannose types | Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| panose + H2O | - |
Aspergillus niger | ? | - |
? | |
| pullulan + H2O | very strict substrate specificity, preferred over panose | Aspergillus niger | isopanose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 59000, deglycosylated recombinant IPU, SDS-PAGE | Aspergillus niger |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| IPU | - |
Aspergillus niger |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 40 | deglycosylated recombinant IPU, native IPU has a similar optimum temperature | Aspergillus niger |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
deglycosylated recombinant IPU, stable up to 40°C, native IPU has a similar thermal stability | Aspergillus niger |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | - |
deglycosylated recombinant IPU, native IPU has a similar pH-optimum | Aspergillus niger |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 7 | deglycosylated recombinant IPU, stable | Aspergillus niger |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
