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Literature summary for 3.2.1.57 extracted from
- Construction of an efficient expression system for Aspergillus isopullulanase in Pichia pastoris, and a simple purification method (2003), Biosci. Biotechnol. Biochem., 67, 1149-1153.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| ipuA gene, expression in Pichia pastoris GS115 | Aspergillus niger |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
strain ATCC 9642 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | recombinant IPU expressed in Pichia pastoris GS115 has a higher carbohydrate content, 41%, than native IPU, 12-15%, or recombinant IPU expressed in Aspergillus oryzae M-2-3, 34% | Aspergillus niger |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant IPU expressed in Pichia pastoris | Aspergillus niger |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 25.2 | - |
pH 3.5, 40°C, recombinant IPU expressed in Pichia pastoris | Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4(2)-alpha-isomaltosylisomaltose + H2O | cleaves the alpha-1,4-glycosidic linkage of the panose motif | Aspergillus niger | ? | - |
? |  |
| 4(3)-alpha-panosylpanose + H2O | cleaves the alpha-1,4-glycosidic linkage of the panose motif | Aspergillus niger | ? | - |
? | |
| 6(2)-alpha-isomaltosylmaltose + H2O | isomaltotriosylglucose, cleaves the alpha-1,4-glycosidic linkage of the panose motif | Aspergillus niger | ? | - |
? | |
| 6(2)-alpha-maltosylmaltose + H2O | cleaves the alpha-1,4-glycosidic linkage of the panose motif | Aspergillus niger | ? | - |
? | |
| 6(3)-alpha-glucosylmaltotriose + H2O | cleaves the alpha-1,4-glycosidic linkage of the panose motif | Aspergillus niger | ? | - |
? | |
| additional information | recombinant IPU hydrolyzes various substrates containing the structure of panose indicating a strict subsite recognition of the panose motif, cleaves the alpha-1,4-glycosidic linkage | Aspergillus niger | ? | - |
? | |
| panose + H2O | cleaves the alpha-1,4-glycosidic linkage | Aspergillus niger | isomaltose + glucose | - |
? | |
| pullulan + H2O | cleaves the alpha-1,4-glycosidic linkage | Aspergillus niger | isopanose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| IPU | - |
Aspergillus niger |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
assay at | Aspergillus niger |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | - |
assay at | Aspergillus niger |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2.4 | 7 | recombinant IPU expressed in Pichia pastoris, at 4°C, retains pullulan-hydrolyzing activity for 12 hours | Aspergillus niger |
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Release 2023.1 (January 2023)
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