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Literature summary for 3.2.1.57 extracted from
- Two components of cell-bound isopullulanase from Aspergillus niger ATCC 9642 - Their purification and enzymatic properties (1996), Biosci. Biotechnol. Biochem., 60, 1795-1798.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Fe3+ | - |
Aspergillus niger |  |
| N-bromosuccinimide | - |
Aspergillus niger | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell bound | - |
Aspergillus niger | - |
- |
| extracellular | - |
Aspergillus niger | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 59000 | - |
F1 and F2 deglycosylated | Aspergillus niger |
| 69000 | - |
F2, SDS-PAGE | Aspergillus niger |
| 71000 | - |
F1, SDS-PAGE | Aspergillus niger |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pullulan + H2O | Aspergillus niger | - |
isopanose + (Glc)4 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
ATCC 9642, IPU F1 and IPU F2 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Aspergillus niger |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus niger |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 27 | - |
F2 | Aspergillus niger |
| 27.3 | - |
F1 | Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-maltosylmaltose + H2O | - |
Aspergillus niger | isopanose + glucose | - |
? | |
| panose + H2O | - |
Aspergillus niger | isomaltose + glucose | - |
? | |
| pullulan + H2O | - |
Aspergillus niger | isopanose + (Glc)4 | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
F2 at pH 3.5 | Aspergillus niger |
| 45 | - |
F1 at pH 3.5 | Aspergillus niger |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
F2, below, 30 min at pH 3.5 | Aspergillus niger |
| 45 | - |
F1, below, 30 min at pH 3.5 | Aspergillus niger |
| 55 | - |
F2, loss of activity | Aspergillus niger |
| 60 | - |
F1, loss of activity | Aspergillus niger |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 4 | - |
Aspergillus niger |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 7 | at 4°C 12 h | Aspergillus niger |
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