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Literature summary for 3.2.1.56 extracted from

  • Su, G.; Li, L.; Huang, H.; Zhong, W.; Yu, P.; Zhang, F.; Linhardt, R.J.
    Production of a low molecular weight heparin using recombinant glycuronidase [corrected] (2015), Carbohydr. Polym., 134, 151-157 .
    View publication on PubMed
Search for more literature for 3.2.1.56

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli TB1 cells and fused with maltose-binding protein Pedobacter heparinus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-D-glucuronosyl-N2,6-disulfo-beta-D-glucosamine + H2O Pedobacter heparinus
-
 D-glucuronate + N2,6-disulfo-D-glucosamine
-
 ?
3-D-glucuronosyl-N2,6-disulfo-beta-D-glucosamine + H2O Pedobacter heparinus ATCC 13125
-
 D-glucuronate + N2,6-disulfo-D-glucosamine
-
 ?

Organism

Organism UniProt Comment Textmining
Pedobacter heparinus
-
 formerly Flavobacterium heparinum
-
Pedobacter heparinus ATCC 13125
-
 formerly Flavobacterium heparinum
-

Purification (Commentary)

Purification (Comment) Organism
amylose resin column chromatography Pedobacter heparinus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-D-glucuronosyl-N2,6-disulfo-beta-D-glucosamine + H2O
-
 Pedobacter heparinus D-glucuronate + N2,6-disulfo-D-glucosamine
-
 ?
3-D-glucuronosyl-N2,6-disulfo-beta-D-glucosamine + H2O
-
 Pedobacter heparinus ATCC 13125 D-glucuronate + N2,6-disulfo-D-glucosamine
-
 ?
heparan sulfate + H2O
-
 Pedobacter heparinus ?
-
 ?
heparan sulfate + H2O
-
 Pedobacter heparinus ATCC 13125 ?
-
 ?
heparin + H2O
-
 Pedobacter heparinus ?
-
 ?
heparin + H2O
-
 Pedobacter heparinus ATCC 13125 ?
-
 ?
heparosan + H2O
-
 Pedobacter heparinus ?
-
 ?
heparosan + H2O
-
 Pedobacter heparinus ATCC 13125 ?
-
 ?

Synonyms

Synonyms Comment Organism
DELTA4,5 unsaturated glycuronidase
-
 Pedobacter heparinus
DELTA4,5DELTA20 unsaturated glycuronidase
-
 Pedobacter heparinus