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Literature summary for 3.2.1.55 extracted from

  • Linares-Pasten, J.A.; Falck, P.; Albasri, K.; Kjellstroem, S.; Adlercreutz, P.; Logan, D.T.; Karlsson, E.N.
    Three-dimensional structures and functional studies of two GH43 arabinofuranosidases from Weissella sp. strain 142 and Lactobacillus brevis (2017), FEBS J., 284, 2019-2036 .
    View publication on PubMed
Search for more literature for 3.2.1.55

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Levilactobacillus brevis
expressed in Escherichia coli BL21(DE3) cells Weissella sp. 142

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
12
-
 1,5-alpha-L-arabinotriose at pH 5.8 and 45°C Levilactobacillus brevis
13
-
 1,5-alpha-L-arabinobiose at pH 5.8 and 45°C Levilactobacillus brevis
18
-
 1,5-alpha-L-arabinotriose at pH 5.8 and 45°C Weissella sp. 142
33
-
 1,5-alpha-L-arabinobiose at pH 5.8 and 45°C Weissella sp. 142

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
88000
-
 gel filtration Weissella sp. 142
181000
-
 gel filtration Levilactobacillus brevis

Organism

Organism UniProt Comment Textmining
Levilactobacillus brevis
-
-
-
Levilactobacillus brevis DSM 1269
-
-
-
Weissella sp. 142 A0A1L7HA00
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,5-alpha-L-arabinobiose + H2O highest activity Levilactobacillus brevis ?
-
 ?
1,5-alpha-L-arabinobiose + H2O highest activity Weissella sp. 142 ?
-
 ?
1,5-alpha-L-arabinobiose + H2O highest activity Levilactobacillus brevis DSM 1269 ?
-
 ?
1,5-alpha-L-arabinotriose + H2O highest activity Levilactobacillus brevis ?
-
 ?
1,5-alpha-L-arabinotriose + H2O high activity Weissella sp. 142 ?
-
 ?
1,5-alpha-L-arabinotriose + H2O highest activity Levilactobacillus brevis DSM 1269 ?
-
 ?
4-nitrophenyl alpha-L-arabinopyranoside + H2O
-
 Levilactobacillus brevis 4-nitrophenol + alpha-L-arabinopyranose
-
 ?
4-nitrophenyl alpha-L-arabinopyranoside + H2O
-
 Weissella sp. 142 4-nitrophenol + alpha-L-arabinopyranose
-
 ?
4-nitrophenyl alpha-L-arabinopyranoside + H2O
-
 Levilactobacillus brevis DSM 1269 4-nitrophenol + alpha-L-arabinopyranose
-
 ?
additional information no activity with 4-nitrophenyl alpha-D-galactopyranoside, 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl beta-D-mannopyranoside, 4-nitrophenyl beta-D-xylopyranoside, 1,3-L-arabino-beta-D-xylobiose, sugar beet arabinan, and rye arabinoxylan Levilactobacillus brevis ?
-
 ?
additional information no activity with 4-nitrophenyl alpha-D-galactopyranoside, 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl beta-D-mannopyranoside, 4-nitrophenyl beta-D-xylopyranoside, 1,3-L-arabino-beta-D-xylobiose, sugar beet arabinan, and rye arabinoxylan Weissella sp. 142 ?
-
 ?
additional information no activity with 4-nitrophenyl alpha-D-galactopyranoside, 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl beta-D-mannopyranoside, 4-nitrophenyl beta-D-xylopyranoside, 1,3-L-arabino-beta-D-xylobiose, sugar beet arabinan, and rye arabinoxylan Levilactobacillus brevis DSM 1269 ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 40000, SDS-PAGE Weissella sp. 142
homodimer 2 * 39768, calculated from amino acid sequence Weissella sp. 142
homotetramer 4 * 40000, SDS-PAGE Levilactobacillus brevis
homotetramer 4 * 39784, calculated from amino acid sequence Levilactobacillus brevis

Synonyms

Synonyms Comment Organism
alpha-L-arabinofuranosidase
-
 Levilactobacillus brevis
alpha-L-arabinofuranosidase
-
 Weissella sp. 142
AraF43
-
 Levilactobacillus brevis
AraF43
-
 Weissella sp. 142

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Levilactobacillus brevis
45
-
-
 Weissella sp. 142

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 the enzyme keeps about 40% of its initial activity after 20 min at 45°C Weissella sp. 142
45
-
 the enzyme loses all activity after 10 min incubation at 45°C and retains about 40% activity after 1 h at 37°C Levilactobacillus brevis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
57
-
 1,5-alpha-L-arabinotriose at pH 5.8 and 45°C Levilactobacillus brevis
71
-
 1,5-alpha-L-arabinobiose at pH 5.8 and 45°C Levilactobacillus brevis
125
-
 1,5-alpha-L-arabinotriose at pH 5.8 and 45°C Weissella sp. 142
221
-
 1,5-alpha-L-arabinobiose at pH 5.8 and 45°C Weissella sp. 142

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8
-
-
 Levilactobacillus brevis
5.8
-
-
 Weissella sp. 142

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.8
-
 1,5-alpha-L-arabinotriose at pH 5.8 and 45°C Levilactobacillus brevis
5.5
-
 1,5-alpha-L-arabinobiose at pH 5.8 and 45°C Levilactobacillus brevis
6.7
-
 1,5-alpha-L-arabinobiose at pH 5.8 and 45°C Weissella sp. 142
6.9
-
 1,5-alpha-L-arabinotriose at pH 5.8 and 45°C Weissella sp. 142