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Literature summary for 3.2.1.55 extracted from
- Structural and functional characterization of a highly secreted alpha-L-arabinofuranosidase (GH62) from Aspergillus nidulans grown on sugarcane bagasse (2017), Biochim. Biophys. Acta, 1865, 1758-1769 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli Arctic cells | Aspergillus nidulans |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| N83Q | the mutant is not glycosylated but shows about wild type activity | Aspergillus nidulans |
| Y312F | the mutant shows significantly reduced activity compared to the wild type enzyme | Aspergillus nidulans |
| Y312S | the mutant shows significantly reduced activity compared to the wild type enzyme | Aspergillus nidulans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus nidulans | Q5AUX2 | - |
- |
| Aspergillus nidulans A773 | Q5AUX2 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | the enzyme is N-glycosylated at asparagine 83 | Aspergillus nidulans |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus nidulans |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture condition:sugarcane bagasse-grown cell | - |
Aspergillus nidulans | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Aspergillus nidulans | 4-nitrophenol + alpha-L-arabinofuranose | - |
? |  |
| 4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Aspergillus nidulans A773 | 4-nitrophenol + alpha-L-arabinofuranose | - |
? | |
| additional information | no activity with debranched arabinan, sugar beet arabinan, linear arabinan, larch arabinogalactan | Aspergillus nidulans | ? | - |
? | |
| additional information | no activity with debranched arabinan, sugar beet arabinan, linear arabinan, larch arabinogalactan | Aspergillus nidulans A773 | ? | - |
? | |
| rye arabinoxylan + H2O | - |
Aspergillus nidulans | arabinose + ? | - |
? | |
| rye arabinoxylan + H2O | - |
Aspergillus nidulans A773 | arabinose + ? | - |
? | |
| wheat arabinoxylan + H2O | - |
Aspergillus nidulans | arabinose + ? | - |
? | |
| wheat arabinoxylan + H2O | - |
Aspergillus nidulans A773 | arabinose + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 35500, SDS-PAGE | Aspergillus nidulans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-L-arabinofuranosidase | - |
Aspergillus nidulans |
| AN7908 | - |
Aspergillus nidulans |
| GH62 | - |
Aspergillus nidulans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 65 | - |
Aspergillus nidulans |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 354.17 | - |
rye arabinoxylan | at pH 5.5 and 60°C | Aspergillus nidulans | |
| 361.74 | - |
wheat arabinoxylan | at pH 5.5 and 60°C | Aspergillus nidulans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Aspergillus nidulans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 6 | - |
Aspergillus nidulans |
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Release 2023.1 (January 2023)
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