Application | Comment | Organism |
---|---|---|
degradation | Abf51A shows greater synergistic effect in combination with xylanase (2.92fold) on wheat arabinoxylan degradation than other reported enzymes, the amounts of arabinose, xylose, and xylobiose are all increased in comparison to that by the enzymes acting individually | Alicyclobacillus sp. |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Alicyclobacillus sp. |
Protein Variants | Comment | Organism |
---|---|---|
E175Q | inactive | Alicyclobacillus sp. |
E194Q | inactive | Alicyclobacillus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 5 mM, 9.6% residual activity | Alicyclobacillus sp. | |
Co2+ | 5 mM, 25.4% residual activity | Alicyclobacillus sp. | |
K+ | 5 mM, 48% residual activity; 5 mM, 58% residual activity | Alicyclobacillus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.46 | - |
4-nitrophenyl alpha-L-arabinofuranoside | pH 6.0, 60°C | Alicyclobacillus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
x * 57000, SDS-PAGE | Alicyclobacillus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alicyclobacillus sp. | A0A0P0ISK8 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Alicyclobacillus sp. | 4-nitrophenol + L-arabinofuranose | - |
? | |
debranched sugar beet arabinan + H2O | - |
Alicyclobacillus sp. | L-arabinose + ? | - |
? | |
additional information | the enzyme exhibits an exo-type mode of action on polyarabinosides by catalyzing the cleavage of alpha-1,2- and alpha-1,3-linked arabinofuranose side chains in sugar beet arabinan and water-soluble wheat arabinoxylan and alpha-1,5-linked arabinofuranosidic bonds in debranched sugar beet arabinan. The enzyme additionally releases xylobiose and xylotriose from wheat arabinoxylan and is active on xylooligosaccharides (xylohexaose 1.2/mM/min, xylopentaose 6.9/mM/min, and xylotetraose 19.7/mM/min), however a lower level of activity | Alicyclobacillus sp. | ? | - |
? | |
sugar beet arabinan + H2O | - |
Alicyclobacillus sp. | L-arabinose + ? | - |
? | |
wheat arabinoxylan + H2O | - |
Alicyclobacillus sp. | L-arabinose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 57000, SDS-PAGE | Alicyclobacillus sp. |
Synonyms | Comment | Organism |
---|---|---|
Abf51A | - |
Alicyclobacillus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Alicyclobacillus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
pH 4-11, stable for 12 h | Alicyclobacillus sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
23 | - |
4-nitrophenyl alpha-L-arabinofuranoside | pH 6.0, 60°C | Alicyclobacillus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Alicyclobacillus sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 11 | 65°C, stable for 12 h | Alicyclobacillus sp. |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
50 | - |
4-nitrophenyl alpha-L-arabinofuranoside | pH 6.0, 60°C | Alicyclobacillus sp. |