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Literature summary for 3.2.1.55 extracted from

  • Amore, A.; Serpico, A.; Amoresano, A.; Vinciguerra, R.; Faraco, V.
    Analysis of the role of O-glycosylation in GH51 alpha-L-arabinofuranosidase from Pleurotus ostreatus (2015), Biotechnol. Appl. Biochem., 62, 727-737.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.55

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Pleurotus ostreatus

Protein Variants

Protein Variants Comment Organism
S160G catalytic properties are slightly changed. The mutation negatively affects the stability of the enzyme at various pHs and temperatures. Circular dichroism analyses show a minimum at 210 nm for wild-type, which is shifted for mutant S160G, suggesting the presence of an unfolded structure, with a role of the glycosylation at the S160 residue for secondary structure stability Pleurotus ostreatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.64
-
 4-nitrophenyl alpha-L-arabinofuranoside wild-type, pH 5.0, 40°C Pleurotus ostreatus
0.89
-
 4-nitrophenyl alpha-L-arabinofuranoside mutant S160G, pH 5.0, 40°C Pleurotus ostreatus
4.1
-
 4-nitrophenyl beta-D-glucopyranoside wild-type, pH 5.0, 40°C Pleurotus ostreatus
5
-
 4-nitrophenyl beta-D-glucopyranoside mutant S160G, pH 5.0, 40°C Pleurotus ostreatus

Organism

Organism UniProt Comment Textmining
Pleurotus ostreatus G0TES6
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,5-alpha-L-arabinohexaose + H2O
-
 Pleurotus ostreatus 1,5-alpha-L-arabinopentaose + L-arabinose
-
 ?
1,5-alpha-L-arabinotriose + H2O
-
 Pleurotus ostreatus 1,5-alpha-L-arabinobiose + L-arabinose
-
 ?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Pleurotus ostreatus 4-nitrophenol + L-arabinofuranose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Pleurotus ostreatus 4-nitrophenol + D-glucose
-
 ?
carboxymethyl linear arabinan + H2O
-
 Pleurotus ostreatus L-arabinose + ?
-
 ?
additional information no substrates: 4-nitrophenyl-beta-D-xylopyranoside, 4-nitrophenyl-alpha-D-glucopyranoside, 2-nitrophenyl-beta-D-galactopyranoside. In presence of AZO-wheat arabinoxylan, the enzyme dispalys an endo-1,4-beta-xylanase activity Pleurotus ostreatus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Pleurotus ostreatus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 half-life for wild-type 16 days, for mutant S160G 7 days Pleurotus ostreatus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.18
-
 4-nitrophenyl beta-D-glucopyranoside mutant S160G, pH 5.0, 40°C Pleurotus ostreatus
0.38
-
 4-nitrophenyl beta-D-glucopyranoside wild-type, pH 5.0, 40°C Pleurotus ostreatus
43
-
 4-nitrophenyl alpha-L-arabinofuranoside mutant S160G, pH 5.0, 40°C Pleurotus ostreatus
50
-
 4-nitrophenyl alpha-L-arabinofuranoside wild-type, pH 5.0, 40°C Pleurotus ostreatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Pleurotus ostreatus

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
 citrate-phosphate buffer, half-life for wild-type 38 days, for mutant S160G 16 days Pleurotus ostreatus