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Literature summary for 3.2.1.55 extracted from

  • Bissaro, B.; Durand, J.; Biarnes, X.; Planas, A.; Monsan, P.; ODonohue, M.; Faure, R.
    Molecular design of non-Leloir furanose-transferring enzymes from an alpha-L-arabinofuranosidase: a rationale for the engineering of evolved transglycosylases (2015), ACS Catal., 5, 4598-4611.
No PubMed abstract available
Search for more literature for 3.2.1.55

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermobacillus xylanilyticus

Protein Variants

Protein Variants Comment Organism
G179F mutant displays stronger xylotriose-mediated inhibition than wild-type Thermobacillus xylanilyticus
L352M 37fold increase of the KM value, mutant displays increased transglycosylation activity (about 18%) Thermobacillus xylanilyticus
N216W almost total absence of inhibition by xylotriose Thermobacillus xylanilyticus
R69H mutant displays severely reduced specific activity and biphasic reaction profile. Transglycosylation activitiy is increased Thermobacillus xylanilyticus
R69H/G179F/L352M mutant displays severely reduced specific activity and biphasic reaction profile and is activated in the presence of xylotriose. Transglycosylation activitiy is increased Thermobacillus xylanilyticus
R69H/L352M activity increases up to 260% in the presence of xylotriose, whereas wild-type is inhibited Thermobacillus xylanilyticus
R69H/N216W/L352M mutant displays severely reduced specific activity and biphasic reaction profile and is activated in the presence of xylotriose. Transglycosylation activitiy is increased Thermobacillus xylanilyticus

Inhibitors

Inhibitors Comment Organism Structure
xylotriose 30 mM, 30% residual activity for wild-type Thermobacillus xylanilyticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09
-
 4-nitrophenyl alpha-L-arabinofuranoside mutant R69H, pH 7.0, 45°C Thermobacillus xylanilyticus
0.25
-
 4-nitrophenyl alpha-L-arabinofuranoside wild-type, pH 7.0, 45°C Thermobacillus xylanilyticus
0.48
-
 4-nitrophenyl alpha-L-arabinofuranoside mutant R69H/N216W/L352M, pH 7.0, 45°C Thermobacillus xylanilyticus
0.69
-
 4-nitrophenyl alpha-L-arabinofuranoside mutant R69H/G179F/L352M, pH 7.0, 45°C Thermobacillus xylanilyticus
1.23
-
 4-nitrophenyl alpha-L-arabinofuranoside mutant R69H/L352M, pH 7.0, 45°C Thermobacillus xylanilyticus
8.98
-
 4-nitrophenyl alpha-L-arabinofuranoside mutant L352M, pH 7.0, 45°C Thermobacillus xylanilyticus

Organism

Organism UniProt Comment Textmining
Thermobacillus xylanilyticus O69262
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Thermobacillus xylanilyticus 4-nitrophenol + L-arabinose
-
 ?

Synonyms

Synonyms Comment Organism
AbjA
-
 Thermobacillus xylanilyticus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.54
-
 4-nitrophenyl alpha-L-arabinofuranoside mutant R69H/G179F/L352M, pH 7.0, 45°C Thermobacillus xylanilyticus
0.58
-
 4-nitrophenyl alpha-L-arabinofuranoside mutant R69H/N216W/L352M, pH 7.0, 45°C Thermobacillus xylanilyticus
0.97
-
 4-nitrophenyl alpha-L-arabinofuranoside mutant R69H/L352M, pH 7.0, 45°C Thermobacillus xylanilyticus
2.29
-
 4-nitrophenyl alpha-L-arabinofuranoside mutant R69H, pH 7.0, 45°C Thermobacillus xylanilyticus
139
-
 4-nitrophenyl alpha-L-arabinofuranoside wild-type, pH 7.0, 45°C Thermobacillus xylanilyticus
233
-
 4-nitrophenyl alpha-L-arabinofuranoside mutant L352M, pH 7.0, 45°C Thermobacillus xylanilyticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8
-
 wild-type Thermobacillus xylanilyticus
6.5
-
 mutants R69H, L3523M, R69H/L352M Thermobacillus xylanilyticus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.8
-
 4-nitrophenyl alpha-L-arabinofuranoside mutant R69H/G179F/L352M, pH 7.0, 45°C Thermobacillus xylanilyticus
0.8
-
 4-nitrophenyl alpha-L-arabinofuranoside mutant R69H/L352M, pH 7.0, 45°C Thermobacillus xylanilyticus
1.2
-
 4-nitrophenyl alpha-L-arabinofuranoside mutant R69H/N216W/L352M, pH 7.0, 45°C Thermobacillus xylanilyticus
25.4
-
 4-nitrophenyl alpha-L-arabinofuranoside mutant R69H, pH 7.0, 45°C Thermobacillus xylanilyticus
25.9
-
 4-nitrophenyl alpha-L-arabinofuranoside mutant L352M, pH 7.0, 45°C Thermobacillus xylanilyticus
556
-
 4-nitrophenyl alpha-L-arabinofuranoside wild-type, pH 7.0, 45°C Thermobacillus xylanilyticus