Cloned (Comment) | Organism |
---|---|
gene poabf, screening of a genetic library after random mutagenesis using error prone PCR system, recombinant expression of wild-type and mutant enzymes in Pichia pastoris using vector pSAL4 | Pleurotus ostreatus |
Protein Variants | Comment | Organism |
---|---|---|
F435Y/Y446F | random mutagenesis, the mutant shows 3fold increased activity and altered substrate specificity and is active with larch arabinogalactan in contrast to the wild-type enzyme | Pleurotus ostreatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pleurotus ostreatus | G0TES6 | gene poabf | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
36.7 | - |
wild-type enzyme, substrate 4-nitrophenyl-alpha-L-arabinofuranoside, pH 5.0, 40°C | Pleurotus ostreatus |
121.7 | - |
recombinant enzyme mutant F435Y/Y446F, substrate 4-nitrophenyl-alpha-L-arabinofuranoside, pH 5.0, 40°C | Pleurotus ostreatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,5-alpha-L-arabinohexaose + H2O | - |
Pleurotus ostreatus | 1,5-alpha-L-arabinopentaose + L-arabinose | - |
? | |
1,5-alpha-L-arabinotriose + H2O | - |
Pleurotus ostreatus | 1,5-alpha-L-arabinobiose + L-arabinose | - |
? | |
4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Pleurotus ostreatus | 4-nitrophenol + L-arabinofuranose | - |
? | |
L-arabinan + H2O | CM-linear arabinan | Pleurotus ostreatus | ? | - |
? | |
larch arabinoxylan + H2O | substrate of mutant F435Y/Y446F, no activity with the wild-type enzyme | Pleurotus ostreatus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GH51 alpha-L-arabinofuranosidase | - |
Pleurotus ostreatus |
PoAbf | - |
Pleurotus ostreatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Pleurotus ostreatus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
purified wild-type and mutant enzymes, pH 5.0, loss of over 90% and 50% activity after 24 h, respectively | Pleurotus ostreatus |
50 | - |
purified wild-type and mutant enzymes, pH 5.0, loss of 75% and 55% activity after 23 h, respectively | Pleurotus ostreatus |
60 | - |
purified wild-type and mutant enzymes, pH 5.0, loss of 75% and 70% activity after 5 min, respectively, almost inactivation after 10 min | Pleurotus ostreatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Pleurotus ostreatus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | - |
purified wild-type and mutant enzymes, 40°C, loss of 60-70% activity after 35 days | Pleurotus ostreatus |
5 | - |
purified wild-type and mutant enzymes, 40°C, completely stable up to 42 days | Pleurotus ostreatus |
7 | 8 | purified wild-type and mutant enzymes, 40°C, loss of 80% activity after 50 days | Pleurotus ostreatus |