Cloned (Comment) | Organism |
---|---|
gene BSU28720, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain BL21(DE3) | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59500 | - |
6 * 59500, recombinant enzyme, SDS-PAGE | Bacillus subtilis |
356000 | - |
recombinant enzyme, native PAGE | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | P94531 | gene BSU28720 | - |
Bacillus subtilis 168 | P94531 | gene BSU28720 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme preferentially cleaves debranched or linear arabinan and is an exo-acting enzyme producing arabinose from arabinoheptaose | Bacillus subtilis | ? | - |
? | |
additional information | the enzyme preferentially cleaves debranched or linear arabinan and is an exo-acting enzyme producing arabinose from arabinoheptaose | Bacillus subtilis 168 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 59500, recombinant enzyme, SDS-PAGE | Bacillus subtilis |
More | the enzyme has a canonical circular dichroism spectrum of alpha/beta proteins and exhibits a hexameric quaternary structure in solution, mechanisms associated with the unfolding process by molecular dynamics simulations, important contribution of the quaternary arrangement in the stabilization of the beta-sandwich accessory domain and other regions involved in the formation of the catalytic interface of hexameric Abfases belonging to GH51 family | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
AbfA | - |
Bacillus subtilis |
alpha-L-Abfase | - |
Bacillus subtilis |
alpha-L-arabinofuranosidase | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 50 | broad optimum | Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
53.5 | - |
melting temperature of the enzyme | Bacillus subtilis |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the glycoside hydrolase 51 family, GH51. Important contribution of the quaternary arrangement in the stabilization of the beta-sandwich accessory domain and other regions involved in the formation of the catalytic interface of hexameric Abfases belonging to GH51 family | Bacillus subtilis |
additional information | structure-function analysis, molecular modeling and molecular dynamic simulations, overview | Bacillus subtilis |