Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.55 extracted from

  • Hoffmam, Z.B.; Oliveira, L.C.; Cota, J.; Alvarez, T.M.; Diogo, J.A.; Neto, M.; Citadini, A.P.; Leite, V.B.; Squina, F.M.; Murakami, M.T.; Ruller, R.
    Characterization of a hexameric exo-acting GH51 alpha-L-arabinofuranosidase from the mesophilic Bacillus subtilis (2013), Mol. Biotechnol., 55, 260-267.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene BSU28720, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain BL21(DE3) Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59500
-
6 * 59500, recombinant enzyme, SDS-PAGE Bacillus subtilis
356000
-
recombinant enzyme, native PAGE Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis P94531 gene BSU28720
-
Bacillus subtilis 168 P94531 gene BSU28720
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme preferentially cleaves debranched or linear arabinan and is an exo-acting enzyme producing arabinose from arabinoheptaose Bacillus subtilis ?
-
?
additional information the enzyme preferentially cleaves debranched or linear arabinan and is an exo-acting enzyme producing arabinose from arabinoheptaose Bacillus subtilis 168 ?
-
?

Subunits

Subunits Comment Organism
hexamer 6 * 59500, recombinant enzyme, SDS-PAGE Bacillus subtilis
More the enzyme has a canonical circular dichroism spectrum of alpha/beta proteins and exhibits a hexameric quaternary structure in solution, mechanisms associated with the unfolding process by molecular dynamics simulations, important contribution of the quaternary arrangement in the stabilization of the beta-sandwich accessory domain and other regions involved in the formation of the catalytic interface of hexameric Abfases belonging to GH51 family Bacillus subtilis

Synonyms

Synonyms Comment Organism
AbfA
-
Bacillus subtilis
alpha-L-Abfase
-
Bacillus subtilis
alpha-L-arabinofuranosidase
-
Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35 50 broad optimum Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
53.5
-
melting temperature of the enzyme Bacillus subtilis

General Information

General Information Comment Organism
evolution the enzyme belongs to the glycoside hydrolase 51 family, GH51. Important contribution of the quaternary arrangement in the stabilization of the beta-sandwich accessory domain and other regions involved in the formation of the catalytic interface of hexameric Abfases belonging to GH51 family Bacillus subtilis
additional information structure-function analysis, molecular modeling and molecular dynamic simulations, overview Bacillus subtilis