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Literature summary for 3.2.1.55 extracted from

  • Goyal, A.; Ahmed, S.; Fontes, C.; Najmudin, S.
    Crystallization and preliminary X-ray crystallographic analysis of a novel alpha-L-arabinofuranosidase (CtGH43) from Clostridium thermocellum ATCC 27405 (2014), Acta Crystallogr. Sect. F, 70, 616-618.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
truncated 903 bp gene encoding the N-terminus of CtGH43 is part of a full-length family 43 glycoside hydrolase Ct43Araf which also contains two tandem family 6 carbohydrate-binding modules, CtCBM6A (405 bp) and CtCBM6B (402 bp), at the C-terminus, recombinant expression of the His6-tagged truncated enzyme in Escherichia coli strain BL21(DE3) pLysS Acetivibrio thermocellus

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant His6-tagged truncated enzyme, sitting drop vapour diffusion method, mixing of 120 mg/ml protein in 50 mM HEPES, pH 7.5, 200 mM NaCl, and 5 mM CaCl2 with precipitant solution containing 0.1 M sodium acetate pH 4.5, 2 M ammonium sulfate and (b) 0.1 M Tris, pH 8.5, 20% PEG 2000 monomethyl ether, 0.2 M trimethyl N-oxide for brick shaped crystals or containing 0.1 M Tris, pH 8.5, 20% PEG 2000 monomethyl ether, 0.2 M trimethyl N-oxide for monoclinic crystals, X-ray diffraction structure determination and analysis at resolution of 1.65 A for the cubic form and 1.1 A for the monoclinic form, molecular replacement method Acetivibrio thermocellus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35860
-
x * 35860, recombinant His6-tagged truncated enzyme, SDS-PAGE Acetivibrio thermocellus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Acetivibrio thermocellus the enzyme in combination with endoxylanase leads to complete breakdown of L-arabinosyl-substituted xylans ?
-
?
additional information Acetivibrio thermocellus ATCC 27405 the enzyme in combination with endoxylanase leads to complete breakdown of L-arabinosyl-substituted xylans ?
-
?

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus A3DIH0
-
-
Acetivibrio thermocellus ATCC 27405 A3DIH0
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged truncated enzyme from Escherichia coli strain BL21(DE3) pLysS by nickel affinity chromatography and gel filtration Acetivibrio thermocellus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme in combination with endoxylanase leads to complete breakdown of L-arabinosyl-substituted xylans Acetivibrio thermocellus ?
-
?
additional information the enzyme in combination with endoxylanase leads to complete breakdown of L-arabinosyl-substituted xylans Acetivibrio thermocellus ATCC 27405 ?
-
?

Subunits

Subunits Comment Organism
? x * 35860, recombinant His6-tagged truncated enzyme, SDS-PAGE Acetivibrio thermocellus

Synonyms

Synonyms Comment Organism
alpha-L-arabinofuranosidase
-
Acetivibrio thermocellus
alpha-L-Araf
-
Acetivibrio thermocellus
CtGH43
-
Acetivibrio thermocellus

General Information

General Information Comment Organism
evolution the enzyme belongs to the glycosyl hydrolase family 43, GH43 Acetivibrio thermocellus