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Literature summary for 3.2.1.55 extracted from

  • Guerfali, M.; Gargouri, A.; Belghith, H.
    Catalytic properties of Talaromyces thermophilus alpha-L-arabinofuranosidase and its synergistic action with immobilized endo-beta-1,4-xylanase (2011), J. Mol. Catal. B, 68, 192-199.
No PubMed abstract available
Search for more literature for 3.2.1.55

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 120% activity at 10 mM Thermomyces dupontii
additional information not influenced by dithiothreitol Thermomyces dupontii
Urea 118% activity at 10 mM Thermomyces dupontii

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 7% residual activity at 5 mM Thermomyces dupontii
EDTA 13% residual activity at 10 mM Thermomyces dupontii
Hg2+ 2% residual activity at 5 mM Thermomyces dupontii
SDS 30% residual activity at 10 mM Thermomyces dupontii
Zn2+ 11% residual activity at 5 mM Thermomyces dupontii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.77
-
 4-nitrophenyl alpha-L-arabinofuranoside in 50 mM potassium phosphate buffer (pH 7.0), at 50°C Thermomyces dupontii

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 133% activity at 5 mM Thermomyces dupontii
Co2+ 123% activity at 5 mM Thermomyces dupontii
Mg2+ 114% activity at 5 mM Thermomyces dupontii
Mn2+ 135% activity at 5 mM Mn2+, the activity of apo-alpha-L-arabinofuranosidase is recovered (48% of reactivation) in the presence of 1 mM Mn2+ Thermomyces dupontii
additional information not influenced by Ba2+ Thermomyces dupontii
additional information the removal of metals from alpha-L-arabinofuranosidase results into almost complete loss of activity and apoenzyme of alpha-L-arabinofuranosidase displays only about 7.6% residual activity Thermomyces dupontii
Na+ 119% activity at 5 mM Thermomyces dupontii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 gel filtration Thermomyces dupontii

Organism

Organism UniProt Comment Textmining
Thermomyces dupontii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Q-sepharose column chromatography, Sephacryl S-200 gel filtration and Mono Q column chromatography Thermomyces dupontii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8
-
 culture supernatant, in 50 mM potassium phosphate buffer (pH 7.0), at 50°C Thermomyces dupontii
49
-
 after 26.7fold purification, in 50 mM potassium phosphate buffer (pH 7.0), at 50°C Thermomyces dupontii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-L-arabinofuranoside + H2O 100% activity Thermomyces dupontii 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
4-nitrophenyl beta-D-mannoside + H2O 3.4% activity compared to 4-nitrophenyl alpha-L-arabinofuranoside Thermomyces dupontii 4-nitrophenol + D-mannose
-
 ?
4-nitrophenyl beta-D-xylopyranoside + H2O 52% activity compared to 4-nitrophenyl alpha-L-arabinofuranoside Thermomyces dupontii 4-nitrophenol + beta-D-xylopyranose
-
 ?
additional information no activity with 4-nitrophenyl alpha-L-arabinopyranoside, 4-nitrophenyl beta-D-glucopyranoside, and 4-nitrophenyl beta-D-cellobioside Thermomyces dupontii ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 35000, SDS-PAGE Thermomyces dupontii

Synonyms

Synonyms Comment Organism
alpha-L-arabinofuranosidase
-
 Thermomyces dupontii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Thermomyces dupontii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 the half-life of the enzyme at 60°C is approximately 2 h. The activity is stable over 7 h at 50°C and is slightly affected at 55°C with a half-life of 4 h. At 70°C, the enzyme is rapidly inactivated and has a half-life of 30 min Thermomyces dupontii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
14.3
-
 4-nitrophenyl alpha-L-arabinofuranoside in 50 mM potassium phosphate buffer (pH 7.0), at 50°C Thermomyces dupontii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7
-
 Thermomyces dupontii

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8 very stable at pH 6.0-8.0 at 4°C, after incubation at pH 6.0, the enzyme is slightly activated relatively to its starting activity, measured under standard conditions. At pH 9.0, alpha-l-arabinofuranosidase retains up to 70% of its initial activity Thermomyces dupontii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
18
-
 4-nitrophenyl alpha-L-arabinofuranoside in 50 mM potassium phosphate buffer (pH 7.0), at 50°C Thermomyces dupontii