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Literature summary for 3.2.1.55 extracted from

  • Lim, Y.R.; Yoon, R.Y.; Seo, E.S.; Kim, Y.S.; Park, C.S.; Oh, D.K.
    Hydrolytic properties of a thermostable alpha-L-arabinofuranosidase from Caldicellulosiruptor saccharolyticus (2010), J. Appl. Microbiol., 109, 1188-1197.
    View publication on PubMed
Search for more literature for 3.2.1.55

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli ER2566 cells Caldicellulosiruptor saccharolyticus

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 92% residual activity at 1 mM Caldicellulosiruptor saccharolyticus
Co2+ 90% residual activity at 1 mM Caldicellulosiruptor saccharolyticus
Cu2+ 79% residual activity at 1 mM Caldicellulosiruptor saccharolyticus
EDTA 90% residual activity at 1 mM Caldicellulosiruptor saccharolyticus
Fe2+ 83% residual activity at 1 mM Caldicellulosiruptor saccharolyticus
Mg2+ 90% residual activity at 1 mM Caldicellulosiruptor saccharolyticus
Mn2+ 97% residual activity at 1 mM Caldicellulosiruptor saccharolyticus
additional information not inhibited by Ba2+ Caldicellulosiruptor saccharolyticus
Zn2+ 94% residual activity at 1 mM Caldicellulosiruptor saccharolyticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1
-
 4-nitrophenyl alpha-L-arabinofuranoside in 50 mM citric acid buffer (pH 5.5), at 80°C Caldicellulosiruptor saccharolyticus
1.29
-
 4-nitrophenyl alpha-L-arabinofuranoside in 50 mM citric acid buffer (pH 5.5), at 80°C Caldicellulosiruptor saccharolyticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57882
-
 8 * 57882, calculated from amino acid sequence Caldicellulosiruptor saccharolyticus
58000
-
 8 * 58000, SDS-PAGE Caldicellulosiruptor saccharolyticus
460000
-
 gel filtration Caldicellulosiruptor saccharolyticus

Organism

Organism UniProt Comment Textmining
Caldicellulosiruptor saccharolyticus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
heat treatment and Hi-Trap anion exchange column chromatography Caldicellulosiruptor saccharolyticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1
-
 crude extract, in 50 mM citric acid buffer (pH 5.5), at 80°C Caldicellulosiruptor saccharolyticus
28.2
-
 after 25.6fold purification, in 50 mM citric acid buffer (pH 5.5), at 80°C Caldicellulosiruptor saccharolyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Caldicellulosiruptor saccharolyticus ?
-
 ?
4-nitrophenyl alpha-L-arabinopyranoside + H2O
-
 Caldicellulosiruptor saccharolyticus ?
-
 ?
additional information no activity towards 4-nitrophenyl alpha-D-galactopyranoside, 4-nitrophenyl alpha-D-glucopyranoside, 4-nitrophenyl beta-D-fucopyranoside, 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl beta-D-lactopyranoside, 4-nitrophenyl beta-D-maltopyranoside, 4-nitrophenyl beta-D-xylopyranoside, 4-nitrophenyl beta-L-arabinopyranoside, 2-nitrophenyl-beta-D-fucopyranoside, 2-nitrophenyl-beta-D-galactopyranoside and 2-nitrophenyl-beta-D-glucopyranoside, starch, arabinan, xylan, debranched arabinan and wheat arabinoxylan Caldicellulosiruptor saccharolyticus ?
-
 ?

Subunits

Subunits Comment Organism
homooctamer 8 * 58000, SDS-PAGE Caldicellulosiruptor saccharolyticus
homooctamer 8 * 57882, calculated from amino acid sequence Caldicellulosiruptor saccharolyticus

Synonyms

Synonyms Comment Organism
alpha-L-arabinofuranosidase
-
 Caldicellulosiruptor saccharolyticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
 Caldicellulosiruptor saccharolyticus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65 80 the half-lives of the enzyme at 65°C, 70°C, 75°C and 80°C were 1700 h, 390 h, 49 h and 0.4 h, respectively Caldicellulosiruptor saccharolyticus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
226
-
 4-nitrophenyl alpha-L-arabinofuranoside in 50 mM citric acid buffer (pH 5.5), at 80°C Caldicellulosiruptor saccharolyticus
285
-
 4-nitrophenyl alpha-L-arabinofuranoside in 50 mM citric acid buffer (pH 5.5), at 80°C Caldicellulosiruptor saccharolyticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Caldicellulosiruptor saccharolyticus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
205
-
 4-nitrophenyl alpha-L-arabinofuranoside in 50 mM citric acid buffer (pH 5.5), at 80°C Caldicellulosiruptor saccharolyticus
221
-
 4-nitrophenyl alpha-L-arabinofuranoside in 50 mM citric acid buffer (pH 5.5), at 80°C Caldicellulosiruptor saccharolyticus