Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)DELTASlyD cells | Thermotoga petrophila |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 100 mM bis-Tris pH 5.5, 200 mM ammonium acetate, 40% (v/v) 2-methyl-2,4 pentanediol and 1% (v/v) dioxane | Thermotoga petrophila |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
x * 57000 | Thermotoga petrophila |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga petrophila | A5IKD0 | - |
- |
Thermotoga petrophila RKU-1 | A5IKD0 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-charged HiTrap Chelating HP column chromatography and Superdex 200 gel filtration | Thermotoga petrophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-1,5-linked arabinoheptaose + H2O | - |
Thermotoga petrophila | L-arabinose + ? | - |
? | |
alpha-1,5-linked arabinoheptaose + H2O | - |
Thermotoga petrophila RKU-1 | L-arabinose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 57000 | Thermotoga petrophila |
Synonyms | Comment | Organism |
---|---|---|
alpha-L-arabinofuranosidase | - |
Thermotoga petrophila |
AraF | - |
Thermotoga petrophila |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Thermotoga petrophila |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 90 | - |
Thermotoga petrophila |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
the enzyme shows no loss of activity after 20 h at 90°C | Thermotoga petrophila |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Thermotoga petrophila |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | - |
Thermotoga petrophila |