Any feedback?
Literature summary for 3.2.1.55 extracted from
- Isolation and characterization of CcAbf62A, a GH62 alpha-L-arabinofuranosidase, from the basidiomycete Coprinopsis cinerea (2011), Biosci. Biotechnol. Biochem., 75, 342-345.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme activity for wheat arabinoxylan is increased 1.47fold by the addition 100000 ng/ml of the carbohydrate esterase Est1 from Coprinus cinerea | Coprinopsis cinerea |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Pichia pastoris cells | Coprinopsis cinerea |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41400 | - |
x * 41400, mature protein, calculated from amino acid sequence | Coprinopsis cinerea |
| 48300 | - |
x * 48300, estimated from SDS-PAGE | Coprinopsis cinerea |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Coprinopsis cinerea | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Coprinopsis cinerea |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA agarose column chromatography | Coprinopsis cinerea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the recombinant enzyme shows no activity with 4-nitrophenyl alpha-L-arabinofuranoside, 4-nitrophenyl alpha-L-arabinopyranoside and 4-nitrophenyl beta-D-xylopyranoside | Coprinopsis cinerea | ? | - |
? |  |
| oat spelt arabinoxylan + H2O | - |
Coprinopsis cinerea | L-arabinose + ? | - |
? | |
| wheat arabinoxylan + H2O | - |
Coprinopsis cinerea | L-arabinose + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 41400, mature protein, calculated from amino acid sequence | Coprinopsis cinerea |
| ? | x * 48300, estimated from SDS-PAGE | Coprinopsis cinerea |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-L-arabinofuranosidase | - |
Coprinopsis cinerea |
| CcAbf62A | a glycoside hydrolase family 62 alpha-L-arabinofuranosidase | Coprinopsis cinerea |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Coprinopsis cinerea |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
the recombinant enzyme retains more than 80% relative activity after 1 h of incubation at pH 7.0-8.5 below 40°C | Coprinopsis cinerea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Coprinopsis cinerea |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8.5 | the recombinant enzyme retains more than 80% relative activity after 1 h of incubation at pH 7.0-8.5 below 40°C | Coprinopsis cinerea |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Coprinopsis cinerea | mature protein, calculated from amino acid sequence | - |
6.8 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
