Any feedback?
Literature summary for 3.2.1.55 extracted from
- Two distinct arabinofuranosidases contribute to arabino-oligosaccharide degradation in Bacillus subtilis (2008), Microbiology, 154, 2719-2729.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli BL21(DE3) | Bacillus subtilis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.36 | - |
linear alpha-1,5-L-arabinan | - |
Bacillus subtilis |  |
| 0.42 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | - |
Bacillus subtilis | |
| 0.49 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | - |
Bacillus subtilis | |
| 0.78 | - |
1,5-alpha-L-arabinobiose | - |
Bacillus subtilis | |
| 1.1 | - |
1,5-alpha-L-arabinotriose | - |
Bacillus subtilis | |
| 2.5 | - |
alpha-1,5-L-arabinotriose | - |
Bacillus subtilis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Bacillus subtilis | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the addition of the chelating agent EDTA does not affect the activity of AbfA, suggesting that no metals are needed for the enzymic reaction | Bacillus subtilis | |
| additional information | the addition of the chelating agent EDTA does not affect the activity of either Abf2, suggesting that no metals are needed for the enzymic reaction | Bacillus subtilis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
2 * 58000, SDS-PAGE | Bacillus subtilis |
| 112000 | - |
Abf2 is present in two oligomeric states, a predominant form with an estimated molecular mass of 112 kDa that could correspond to the dimer and an additional oligomeric form of about 276 kDa | Bacillus subtilis |
| 116000 | - |
gradient native PAGE, two oligomeric forms are observed, the dimer (116 kDa) and an oligomer with a higher-order structure than the tetramer | Bacillus subtilis |
| 276000 | - |
Abf2 is present in two oligomeric states, a predominant form with an estimated molecular mass of 112 kDa that could correspond to the dimer and an additional oligomeric form of about 276 kDa | Bacillus subtilis |
| 296000 | - |
gradient native PAGE, two oligomeric forms are observed, the dimer (116 kDa) and an oligomer with a higher-order structure than the tetramer | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | P94531 | - |
- |
| Bacillus subtilis | P94552 | - |
- |
| Bacillus subtilis 168T+ | P94531 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA agarose affinity chromatography | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,5-alpha-L-arabinobiose + H2O | - |
Bacillus subtilis | ? | - |
? | |
| 1,5-alpha-L-arabinobiose + H2O | - |
Bacillus subtilis 168T+ | ? | - |
? | |
| 1,5-alpha-L-arabinotriose + H2O | - |
Bacillus subtilis | ? | - |
? | |
| 1,5-alpha-L-arabinotriose + H2O | - |
Bacillus subtilis 168T+ | ? | - |
? | |
| alpha-1,5-L-arabinotriose + H2O | - |
Bacillus subtilis | ? | - |
? | |
| linear alpha-1,5-L-arabinan + H2O | - |
Bacillus subtilis | ? | - |
? | |
| linear alpha-1,5-L-arabinan + H2O | - |
Bacillus subtilis 168T+ | ? | - |
? | |
| additional information | Abf2 is most active on (1-2) and (1-3) linkages of branched arabinan and arabinoxylan | Bacillus subtilis | ? | - |
? | |
| additional information | AbfA acts preferentially on (1-5) linkages of linear alpha-1,5-L-arabinan and alpha-1,5-linked arabino-oligomers, and is much less effective on branched sugar beet arabinan and arabinoxylan and arabinogalactan | Bacillus subtilis | ? | - |
? | |
| additional information | AbfA acts preferentially on (1-5) linkages of linear alpha-1,5-L-arabinan and alpha-1,5-linked arabino-oligomers, and is much less effective on branched sugar beet arabinan and arabinoxylan and arabinogalactan | Bacillus subtilis 168T+ | ? | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Bacillus subtilis | p-nitrophenol + L-arabinofuranose | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Bacillus subtilis 168T+ | p-nitrophenol + L-arabinofuranose | - |
? | |
| sugar beet arabinan (branched) + H2O | - |
Bacillus subtilis | ? | - |
? | |
| wheat arabinoxylan + H2O | - |
Bacillus subtilis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 58000, SDS-PAGE | Bacillus subtilis |
| oligomer | higher than the tetramer, subunit mass 58000 | Bacillus subtilis |
| oligomer | two oligomeric forms are observed, the dimer (116 kDa) and an oligomer with a higher-order structure than the tetramer, subunit mass 58000 | Bacillus subtilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Abf2 | - |
Bacillus subtilis |
| AbfA | - |
Bacillus subtilis |
| AFS | - |
Bacillus subtilis |
| alpha-L-arabinofuranosidase | - |
Bacillus subtilis |
| alpha-L-arabinofuranoside arabinofuranohydrolase | - |
Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Bacillus subtilis |
| 60 | - |
- |
Bacillus subtilis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 80 | - |
Bacillus subtilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
AbfA has a half-life of about 17.6 min at 55°C | Bacillus subtilis |
| 70 | - |
Abf2 has a half-life of about 9.34 min at 70°C | Bacillus subtilis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.75 | - |
1,5-alpha-L-arabinotriose | - |
Bacillus subtilis | |
| 12 | - |
linear alpha-1,5-L-arabinan | - |
Bacillus subtilis | |
| 32 | - |
1,5-alpha-L-arabinobiose | - |
Bacillus subtilis | |
| 88 | - |
1,5-alpha-L-arabinotriose | - |
Bacillus subtilis | |
| 295 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | - |
Bacillus subtilis | |
| 306 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | - |
Bacillus subtilis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Bacillus subtilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 9 | - |
Bacillus subtilis |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
