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Literature summary for 3.2.1.55 extracted from
- Cloning, purification, and characterization of a thermostable alpha-L-arabinofuranosidase from Anoxybacillus kestanbolensis AC26Sari (2008), Appl. Microbiol. Biotechnol., 81, 61-68.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli BL21 | Anoxybacillus kestanbolensis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | strong inhibition | Anoxybacillus kestanbolensis |  |
| Hg2+ | strong inhibition | Anoxybacillus kestanbolensis | |
| additional information | activity of the enzyme is not affected by the chelating agent EDTA (10 mM) or by the reducing agents DTT and beta mercaptoethanol (10 mM) | Anoxybacillus kestanbolensis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.139 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | - |
Anoxybacillus kestanbolensis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | metals with a small stimulating effect on the activity are Zn2+, Mg2+, and Ca2+, no effect with Co2+, Mn2+ | Anoxybacillus kestanbolensis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
histidine-tagged AbfAC26Sari, SDS-PAGE | Anoxybacillus kestanbolensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Anoxybacillus kestanbolensis | B2D088 | - |
- |
| Anoxybacillus kestanbolensis AC26Sari | B2D088 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| MagneHisTM Protein Purification System | Anoxybacillus kestanbolensis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.31 | - |
crude extract | Anoxybacillus kestanbolensis |
| 1112 | - |
HisTaq purified enzyme | Anoxybacillus kestanbolensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,5-alpha-L-arabinobiose + H2O | - |
Anoxybacillus kestanbolensis | L-arabinose + ? | - |
? | |
| 1,5-alpha-L-arabinopentaose + H2O | - |
Anoxybacillus kestanbolensis | L-arabinose | - |
? | |
| 1,5-alpha-L-arabinotetraose + H2O | - |
Anoxybacillus kestanbolensis | L-arabinose | - |
? | |
| 1,5-alpha-L-arabinotetraose + H2O | - |
Anoxybacillus kestanbolensis AC26Sari | L-arabinose | - |
? | |
| 1,5-alpha-L-arabinotriose + 2 H2O | - |
Anoxybacillus kestanbolensis | 3 L-arabinose | - |
? | |
| 1,5-alpha-L-arabinotriose + 2 H2O | - |
Anoxybacillus kestanbolensis AC26Sari | 3 L-arabinose | - |
? | |
| arabinan + H2O | - |
Anoxybacillus kestanbolensis | L-arabinose + ? | - |
? | |
| arabinoxylan + H2O | - |
Anoxybacillus kestanbolensis | L-arabinose + ? | - |
? | |
| additional information | no activity with a variety of p-nitrophenyl-glycosides, including those with beta-linkages and pyranoside conformations of arabinose | Anoxybacillus kestanbolensis | ? | - |
? | |
| additional information | no activity with a variety of p-nitrophenyl-glycosides, including those with beta-linkages and pyranoside conformations of arabinose | Anoxybacillus kestanbolensis AC26Sari | ? | - |
? | |
| oat spelt xylan + H2O | - |
Anoxybacillus kestanbolensis | L-arabinose + ? | - |
? | |
| oat spelt xylan + H2O | - |
Anoxybacillus kestanbolensis AC26Sari | L-arabinose + ? | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Anoxybacillus kestanbolensis | p-nitrophenol + L-arabinose | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Anoxybacillus kestanbolensis AC26Sari | p-nitrophenol + L-arabinose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-L-arabinofuranosidase | - |
Anoxybacillus kestanbolensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Anoxybacillus kestanbolensis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 85 | - |
Anoxybacillus kestanbolensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 85 | the enzyme maintains full activity at 50°C, 96% activity at 55°C, 60°C, and 65°C, and 80% activity at 70°C for 24 h | Anoxybacillus kestanbolensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Anoxybacillus kestanbolensis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 9 | - |
Anoxybacillus kestanbolensis |
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