Cloned (Comment) | Organism |
---|---|
a histidine tag is introduced at the N-terminal end of AbfATK4, and the recombinant protein is expressed in Escherichia coli BL21, under control of isopropyl-beta-D-thiogalactopyranoside-inducible T7 promoter | Parageobacillus caldoxylosilyticus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 1 mM, complete inhibition | Parageobacillus caldoxylosilyticus | |
Hg2+ | 1 mM, complete inhibition | Parageobacillus caldoxylosilyticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | - |
Parageobacillus caldoxylosilyticus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Parageobacillus caldoxylosilyticus | - |
- |
intracellular | - |
Parageobacillus caldoxylosilyticus | 5622 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | stimulates | Parageobacillus caldoxylosilyticus | |
Mn2+ | stimulates | Parageobacillus caldoxylosilyticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59000 | - |
4 * 59000, SDS-PAGE | Parageobacillus caldoxylosilyticus |
236000 | - |
gel filtration | Parageobacillus caldoxylosilyticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Parageobacillus caldoxylosilyticus | - |
- |
- |
Parageobacillus caldoxylosilyticus TK4 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Parageobacillus caldoxylosilyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no endoarabinanase activity | Parageobacillus caldoxylosilyticus | ? | - |
? | |
additional information | no endoarabinanase activity | Parageobacillus caldoxylosilyticus TK4 | ? | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Parageobacillus caldoxylosilyticus | p-nitrophenol + L-arabinofuranose | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Parageobacillus caldoxylosilyticus TK4 | p-nitrophenol + L-arabinofuranose | - |
? | |
p-nitrophenyl-beta-D-xylopyranoside + H2O | low activity | Parageobacillus caldoxylosilyticus | p-nitrophenol + beta-D-xylopyranose | - |
? | |
p-nitrophenyl-beta-D-xylopyranoside + H2O | low activity | Parageobacillus caldoxylosilyticus TK4 | p-nitrophenol + beta-D-xylopyranose | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 59000, SDS-PAGE | Parageobacillus caldoxylosilyticus |
Synonyms | Comment | Organism |
---|---|---|
AbfATK4 | - |
Parageobacillus caldoxylosilyticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | 80 | - |
Parageobacillus caldoxylosilyticus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 65 | 96 h, stable | Parageobacillus caldoxylosilyticus |
70 | - |
48 h, stable | Parageobacillus caldoxylosilyticus |
75 | - |
12 h, stable | Parageobacillus caldoxylosilyticus |
80 | - |
30 min, 10% loss of activity. Complete loss of activity after 80 min | Parageobacillus caldoxylosilyticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Parageobacillus caldoxylosilyticus |