Cloned (Comment) | Organism |
---|---|
expression in Aspergillus oryzae, GH43 alpha-L-arabinofuranosidase | Humicola insolens |
GH51 alpha-L-arabinofuranosidase | Humicola insolens |
GH51 alpha-L-arabinofuranosidase | Meripilus giganteus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
x * 62000, GH43 alpha-L-arabinofuranosidase, SDS-PAGE | Humicola insolens |
69000 | - |
x * 69000, GH51 alpha-L-arabinofuranosidase, SDS-PAGE | Meripilus giganteus |
94000 | - |
x * 94000, GH51 alpha-L-arabinofuranosidase, SDS-PAGE | Humicola insolens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bifidobacterium sp. | - |
- |
- |
Humicola insolens | - |
- |
- |
Meripilus giganteus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
GH43 alpha-L-arabinofuranosidase | Humicola insolens |
GH51 alpha-L-arabinofuranosidase | Humicola insolens |
GH51 alpha-L-arabinofuranosidase | Meripilus giganteus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-L-arabinofuranoside + H2O | GH51 alpha-L-arabinofuranosidase | Meripilus giganteus | 4-nitrophenol + L-arabinofuranose | - |
? | |
arabinoxylan + H2O | GH43 enzyme selectively hydrolyses (1,3)-alpha-L-arabinofuranosyl residues of doubly substituted xylopyranosyl residues in arabinoxylan and in arabinoxylan-derived oligosaccharides. Combinations (50:50) of the GH43 alpha-AraF from Bifidobacterium sp. and the GH51 alpha-AraFs from Meripilus giganteus or Humicola insolens results in a synergistic increase in arabinose release from water-soluble wheat arabinoxylan | Bifidobacterium sp. | ? | - |
? | |
arabinoxylan + H2O | GH43 enzyme selectively hydrolyses (1,3)-alpha-L-arabinofuranosyl residues of doubly substituted xylopyranosyl residues in arabinoxylan and in arabinoxylan-derived oligosaccharides. Combinations (50:50) of the GH43 alpha-AraF from Humicola insolens and the GH51 alpha-AraFs from Meripilus giganteus or Humicola insolens results in a synergistic increase in arabinose release from water-soluble wheat arabinoxylan in extended reactions at pH 6 and 40°C | Humicola insolens | ? | - |
? | |
arabinoxylan + H2O | GH51 enzyme catalyses removal of (1,2) and (1,3)-alpha-L-arabinofuranosyl residues from single substituted xylopyranosyls in arabinoxylan. Combinations (50:50) of the GH43 alpha-AraF from Humicola insolens and the GH51 alpha-AraFs from Meripilus giganteus results in a synergistic increase in arabinose release from water-soluble wheat arabinoxylan in extended reactions at pH 6 and 40°C | Meripilus giganteus | ? | - |
? | |
arabinoxylan + H2O | GH51 enzyme catalyses removal of (1,2) and (1,3)-alpha-L-arabinofuranosyl residues from singly substituted xylopyranosyls in arabinoxylan. Combinations (50:50) of the GH43 alpha-AraF from Humicola insolens and the GH51 alpha-AraFs from Meripilus giganteus results in a synergistic increase in arabinose release from water-soluble wheat arabinoxylan in extended reactions at pH 6 and 40°C | Humicola insolens | ? | - |
? | |
arabinoxylo-oligosaccharides + H2O | GH43 enzyme selectively hydrolysed (1,3)-alpha-L-arabinofuranosyl residues of doubly substituted xylopyranosyl residues in arabinoxylan and in arabinoxylan-derived oligosaccharides | Humicola insolens | ? | - |
? | |
arabinoxylo-oligosaccharides + H2O | GH43 enzyme selectively hydrolysed (1,3)-alpha-L-arabinofuranosyl residues of doubly substituted xylopyranosyl residues in arabinoxylan and in arabinoxylan-derived oligosaccharides | Bifidobacterium sp. | ? | - |
? | |
arabinoxylo-oligosaccharides + H2O | GH51 enzyme catalyses removal of (1,2) and (1,3)-alpha-L-arabinofuranosyl residues from singly substituted xylopyranosyls in arabinoxylan | Humicola insolens | ? | - |
? | |
arabinoxylo-oligosaccharides + H2O | GH51 enzyme catalyses removal of (1,2) and (1,3)-alpha-L-arabinofuranosyl residues from singly substituted xylopyranosyls in arabinoxylan | Meripilus giganteus | ? | - |
? | |
additional information | no activity with p-nitrophenyl-alpha-L-arabinofuranoside, GH43 alpha-L-arabinofuranosidase | Humicola insolens | ? | - |
? | |
additional information | no activity with p-nitrophenyl-alpha-L-arabinofuranoside, GH43 alpha-L-arabinofuranosidase | Bifidobacterium sp. | ? | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | GH51 alpha-L-arabinofuranosidase | Humicola insolens | p-nitrophenol + L-arabinofuranose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 62000, GH43 alpha-L-arabinofuranosidase, SDS-PAGE | Humicola insolens |
? | x * 69000, GH51 alpha-L-arabinofuranosidase, SDS-PAGE | Meripilus giganteus |
? | x * 94000, GH51 alpha-L-arabinofuranosidase, SDS-PAGE | Humicola insolens |
Synonyms | Comment | Organism |
---|---|---|
alpha-AraF | - |
Humicola insolens |
alpha-AraF | - |
Bifidobacterium sp. |
alpha-AraF | - |
Meripilus giganteus |
GH43 alpha-L-arabinofuranosidase | - |
Humicola insolens |
GH43 alpha-L-arabinofuranosidase | - |
Bifidobacterium sp. |
GH51 alpha-L-arabinofuranosidase | - |
Humicola insolens |
GH51 alpha-L-arabinofuranosidase | - |
Meripilus giganteus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
release of arabinose from water-soluble wheat arabinoxylan, GH43 alpha-L-arabinofuranosidase. Arabinose release catalysed by the Bifidobacterium sp. GH43 enzyme is almost nil outside the exact optimum | Bifidobacterium sp. |
48 | - |
release of arabinose from water-soluble wheat arabinoxylan, GH51 alpha-L-arabinofuranosidase | Meripilus giganteus |
53 | - |
release of arabinose from water-soluble wheat arabinoxylan, GH43 alpha-L-arabinofuranosidase | Humicola insolens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.8 | - |
release of arabinose from water-soluble wheat arabinoxylan, GH51 alpha-L-arabinofuranosidase | Meripilus giganteus |
6 | - |
release of arabinose from water-soluble wheat arabinoxylan, GH43 alpha-L-arabinofuranosidase. Arabinose release catalysed by the Bifidobacterium sp. GH43 enzyme is almost nil outside the exact optimum | Bifidobacterium sp. |
6 | - |
release of arabinose from water-soluble wheat arabinoxylan, GH51 alpha-L-arabinofuranosidase | Humicola insolens |
6.7 | - |
release of arabinose from water-soluble wheat arabinoxylan, GH43 alpha-L-arabinofuranosidase | Humicola insolens |