Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli BL21(DE3) | Thermobacillus xylanilyticus |
Protein Variants | Comment | Organism |
---|---|---|
D55A | site-directed mutagenesis, activity is similar to wild-type enzyme activity | Thermobacillus xylanilyticus |
E112A | site-directed mutagenesis, activity is similar to wild-type enzyme activity | Thermobacillus xylanilyticus |
E143A | site-directed mutagenesis, activity is similar to wild-type enzyme activity | Thermobacillus xylanilyticus |
E176A | site-directed mutagenesis, exchange of the acid-base residue, nearly inactive mutant, 8925fold reduced activity compared to the wild-type enzyme | Thermobacillus xylanilyticus |
E176D | site-directed mutagenesis, exchange of the acid-base residue, mutant enzyme shows altered kinetic properties compared to the wild-type enzyme | Thermobacillus xylanilyticus |
E176Q | site-directed mutagenesis, exchange of the acid-base residue, mutant enzyme is insensitive to pH | Thermobacillus xylanilyticus |
E28A | site-directed mutagenesis, nearly inactive mutant, 5950fold reduced activity compared to the wild-type enzyme | Thermobacillus xylanilyticus |
E28A/E176A | site-directed mutagenesis, completely inactive mutant | Thermobacillus xylanilyticus |
E28D | site-directed mutagenesis, mutant enzyme shows altered kinetic properties compared to the wild-type enzyme | Thermobacillus xylanilyticus |
E28Q | site-directed mutagenesis, mutant enzyme shows altered kinetic properties compared to the wild-type enzyme | Thermobacillus xylanilyticus |
E28Q | site-directed mutagenesis, mutant enzyme shows increased sensitivity to pH and a lower pH optimum compared to the wild-type enzyme | Thermobacillus xylanilyticus |
E298A | site-directed mutagenesis, nearly inactive mutant, 178500fold reduced activity compared to the wild-type enzyme | Thermobacillus xylanilyticus |
E298D | site-directed mutagenesis, mutant enzyme shows altered kinetic properties compared to the wild-type enzyme | Thermobacillus xylanilyticus |
E298Q | site-directed mutagenesis, mutant enzyme shows increased sensitivity to pH and a lower pH optimum compared to the wild-type enzyme | Thermobacillus xylanilyticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, wild-type and mutant enzymes, influence of sodium formate and sodium azide, overview | Thermobacillus xylanilyticus | |
0.3 | - |
4-nitrophenyl-alpha-L-arabinofuranoside | pH 5.8, 60°C, mutant E176D | Thermobacillus xylanilyticus | |
0.41 | - |
4-nitrophenyl-alpha-L-arabinofuranoside | pH 5.8, 60°C, mutant E176Q | Thermobacillus xylanilyticus | |
0.43 | - |
4-nitrophenyl-alpha-L-arabinofuranoside | pH 5.8, 60°C, mutant E176A | Thermobacillus xylanilyticus | |
0.51 | - |
4-nitrophenyl-alpha-L-arabinofuranoside | pH 5.8, 60°C, mutant E28D | Thermobacillus xylanilyticus | |
0.7 | - |
4-nitrophenyl-alpha-L-arabinofuranoside | pH 5.8, 60°C, wild-type enzyme | Thermobacillus xylanilyticus | |
0.91 | - |
4-nitrophenyl-alpha-L-arabinofuranoside | pH 5.8, 60°C, mutant E298Q | Thermobacillus xylanilyticus | |
1.04 | - |
4-nitrophenyl-alpha-L-arabinofuranoside | pH 5.8, 60°C, mutant E28A | Thermobacillus xylanilyticus | |
1.11 | - |
4-nitrophenyl-alpha-L-arabinofuranoside | pH 5.8, 60°C, mutant E298D | Thermobacillus xylanilyticus | |
34 | - |
4-nitrophenyl-alpha-L-arabinofuranoside | pH 5.8, 60°C, mutant E28Q | Thermobacillus xylanilyticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Sodium azide | activates mutant enzymes: E28A 6fold, E176A 7fold, and E298A 67fold, at up to 0.5 M, no activation of the wild-type enzyme | Thermobacillus xylanilyticus | |
Sodium formate | activates mutant enzymes: E28A 60fold at 4 M, E176A 4fold at 4 M, and E298A 36fold at 0.5-1.0 M, no activation of the wild-type enzyme | Thermobacillus xylanilyticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56102 | - |
x * 56102, sequence calculation | Thermobacillus xylanilyticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermobacillus xylanilyticus | O69262 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Escherichia coli | Thermobacillus xylanilyticus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
1,5-alpha-L-arabinofuranohexaose + 5 H2O = 6 alpha-L-arabinofuranose | reaction mechanism, active site structure, Glu28, the catalytic nucleophil, Glu176, the acid-base residue, and Glu298, responsible for modulation of the ionization state of the acid-base and for substrate fixation, are important for catalytic activity | Thermobacillus xylanilyticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
347 | - |
purified recombinant mutant D55A | Thermobacillus xylanilyticus |
356 | - |
purified recombinant mutant E112A | Thermobacillus xylanilyticus |
357 | - |
purified recombinant wild-type enzyme | Thermobacillus xylanilyticus |
361 | - |
purified recombinant mutant E176A | Thermobacillus xylanilyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-L-arabinofuranoside + H2O | hydrolysis of the substrate with retention of the anomeric configuration | Thermobacillus xylanilyticus | 4-nitrophenol + alpha-L-arabinofuranose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 56102, sequence calculation | Thermobacillus xylanilyticus |
Synonyms | Comment | Organism |
---|---|---|
alpha-L-arabinofuranosidase D3 | - |
Thermobacillus xylanilyticus |
More | enzyme belongs to family 51 of the glycosyl hydrolase classification as part of the 4/7 glycosyl hydrolase superfamily | Thermobacillus xylanilyticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
assay at | Thermobacillus xylanilyticus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.39 | - |
4-nitrophenyl-alpha-L-arabinofuranoside | pH 5.8, 60°C, mutant E176A | Thermobacillus xylanilyticus | |
0.53 | - |
4-nitrophenyl-alpha-L-arabinofuranoside | pH 5.8, 60°C, mutant E298D | Thermobacillus xylanilyticus | |
0.63 | - |
4-nitrophenyl-alpha-L-arabinofuranoside | pH 5.8, 60°C, mutant E28A | Thermobacillus xylanilyticus | |
0.65 | - |
4-nitrophenyl-alpha-L-arabinofuranoside | pH 5.8, 60°C, mutant E176D | Thermobacillus xylanilyticus | |
1.04 | - |
4-nitrophenyl-alpha-L-arabinofuranoside | pH 5.8, 60°C, mutant E298Q | Thermobacillus xylanilyticus | |
1.08 | - |
4-nitrophenyl-alpha-L-arabinofuranoside | pH 5.8, 60°C, mutant E28D | Thermobacillus xylanilyticus | |
5.95 | - |
4-nitrophenyl-alpha-L-arabinofuranoside | pH 5.8, 60°C, mutant E28Q | Thermobacillus xylanilyticus | |
36.7 | - |
4-nitrophenyl-alpha-L-arabinofuranoside | pH 5.8, 60°C, mutant E176Q | Thermobacillus xylanilyticus | |
4100 | - |
4-nitrophenyl-alpha-L-arabinofuranoside | pH 5.8, 60°C, wild-type enzyme | Thermobacillus xylanilyticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.6 | 6.2 | - |
Thermobacillus xylanilyticus |