Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, encoded on chromosome 2H | Hordeum vulgare |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67000 | - |
x * 67000, SDS-PAGE, x * 79200, about, amino acid sequence calculation | Hordeum vulgare |
79200 | - |
x * 67000, SDS-PAGE, x * 79200, about, amino acid sequence calculation | Hordeum vulgare |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hordeum vulgare | Q8W012 | bifunctional enzyme | - |
Purification (Comment) | Organism |
---|---|
1080fold from 5 days old seedlings | Hordeum vulgare |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
1,5-alpha-L-arabinofuranohexaose + 5 H2O = 6 alpha-L-arabinofuranose | enzyme shows 2 catalytic activities: alpha-L-arabinofuranosidase and beta-D-xylosidase activity | Hordeum vulgare |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | young | Hordeum vulgare | - |
root | young | Hordeum vulgare | - |
seedling | - |
Hordeum vulgare | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6610 | - |
purified enzyme | Hordeum vulgare |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(1-4)-beta-D-xylopentaose + H2O | - |
Hordeum vulgare | beta-D-xylose | - |
? | |
(1-5)-alpha-L-arabinofuranohexaose + H2O | - |
Hordeum vulgare | alpha-L-arabinofuranose | - |
? | |
4-nitrophenyl alpha-L-arabinofuranoside + H2O | best substrate | Hordeum vulgare | 4-nitrophenol + alpha-L-arabinofuranose | - |
? | |
4-nitrophenyl beta-D-galactopyranoside + H2O | 16% activity compared to 4-nitrophenyl-alpha-L-arabinofuranoside | Hordeum vulgare | 4-nitrophenol + beta-D-galactopyranose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | 20% activity compared to 4-nitrophenyl-alpha-L-arabinofuranoside | Hordeum vulgare | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
4-nitrophenyl-alpha-L-arabinopyranoside + H2O | 11% activity compared to 4-nitrophenyl-alpha-L-arabinofuranoside | Hordeum vulgare | 4-nitrophenol + alpha-L-arabinopyranose | - |
? | |
additional information | stereospecific, enzyme shows 2 catalytic activities: alpha-L-arabinofuranosidase and beta-D-xylosidase activity, no activity with 4-nitrophenyl-beta-D-glucopyranoside, 4-nitrophenyl-alpha-D-glucopyranoside, 4-nitrophenyl-alpha-D-galactopyranoside, 4-nitrophenyl-beta-D-mannopyranoside, 4-nitrophenyl-alpha-D-mannopyranoside, 4-nitrophenyl-alpha-D-xylopyranoside, 4-nitrophenyl-beta-L-arabinopyranoside | Hordeum vulgare | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 67000, SDS-PAGE, x * 79200, about, amino acid sequence calculation | Hordeum vulgare |
Synonyms | Comment | Organism |
---|---|---|
alpha-L-arabinofuranosidase | - |
Hordeum vulgare |
ARA-I | - |
Hordeum vulgare |
More | enzyme belongs to family 3 of glycoside hydrolases | Hordeum vulgare |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.7 | - |
- |
Hordeum vulgare |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Hordeum vulgare | isoelectric focusing | - |
5.5 |