Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 92% inhibition of AFQ1, 89% inhibition of AFS, at 1 mM | Penicillium chrysogenum | |
Cd2+ | 37% inhibition of AFQ1, 15% inhibition of AFS, at 1 mM | Penicillium chrysogenum | |
Cu2+ | 76% inhibition of AFQ1, 36% inhibition of AFS, at 1 mM | Penicillium chrysogenum | |
Hg2+ | 90% inhibition of AFQ1, 74% inhibition of AFS, at 1 mM | Penicillium chrysogenum | |
L-arabinose | 10 mg/ml, inhibition of enzyme AFS1, not AFQ1 | Penicillium chrysogenum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.86 | - |
4-nitrophenyl-alpha-L-arabinofuranoside | enzyme AFQ1, pH 5.0, 37°C | Penicillium chrysogenum | |
1.32 | - |
4-nitrophenyl-alpha-L-arabinofuranoside | enzyme AFS1, pH 5.0, 37°C | Penicillium chrysogenum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Penicillium chrysogenum | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
type B enzyme AFS1, gel filtration | Penicillium chrysogenum |
52000 | - |
x * 79000, type B enzyme AFQ1, x * 52000, type B enzyme AFS1 | Penicillium chrysogenum |
71000 | - |
type B enzyme AFQ1, gel filtration | Penicillium chrysogenum |
79000 | - |
x * 79000, type B enzyme AFQ1, x * 52000, type B enzyme AFS1 | Penicillium chrysogenum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Penicillium chrysogenum | - |
strain 31B, 2 distinct type B enzymes encoded by different genes | - |
Penicillium chrysogenum 31B | - |
strain 31B, 2 distinct type B enzymes encoded by different genes | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | enzyme AFQ1 is a glycoprotein, while enzyme AFS1 is not | Penicillium chrysogenum |
no glycoprotein | AFS1 | Penicillium chrysogenum |
Purification (Comment) | Organism |
---|---|
both type B enzymes AFQ1, 17fold, and AFS1, 39.7fold, from culture filtrate | Penicillium chrysogenum |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.8 | - |
purified enzyme AFQ1 | Penicillium chrysogenum |
13.5 | - |
purified enzyme AFS1 | Penicillium chrysogenum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Penicillium chrysogenum | 4-nitrophenol + alpha-L-arabinofuranose | - |
? | |
4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Penicillium chrysogenum 31B | 4-nitrophenol + alpha-L-arabinofuranose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | low activity only of enzyme AFS1, not AFQ1 | Penicillium chrysogenum | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | low activity only of enzyme AFS1, not AFQ1 | Penicillium chrysogenum 31B | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
arabino-oligosaccharides + H2O | - |
Penicillium chrysogenum | alpha-L-arabinose | - |
? | |
arabino-oligosaccharides + H2O | - |
Penicillium chrysogenum 31B | alpha-L-arabinose | - |
? | |
arabinogalactan + H2O | from soybean | Penicillium chrysogenum | alpha-L-arabinose + ? | - |
? | |
arabinogalactan + H2O | from soybean | Penicillium chrysogenum 31B | alpha-L-arabinose + ? | - |
? | |
arabinoxylan + H2O | from wheat | Penicillium chrysogenum | alpha-L-arabinose + ? | - |
? | |
branched L-arabinan + H2O | from sugar beet | Penicillium chrysogenum | L-arabinose + ? | - |
? | |
debranched arabinan + H2O | from sugar beet | Penicillium chrysogenum | alpha-L-arabinose + ? | - |
? | |
additional information | both type B enzymes show broad substrate specificity, synergistic action between AFQ1, AFS1, and exo-arabinanase in degradation of arabinan | Penicillium chrysogenum | ? | - |
? | |
additional information | both type B enzymes show broad substrate specificity, synergistic action between AFQ1, AFS1, and exo-arabinanase in degradation of arabinan | Penicillium chrysogenum 31B | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 79000, type B enzyme AFQ1, x * 52000, type B enzyme AFS1 | Penicillium chrysogenum |
Synonyms | Comment | Organism |
---|---|---|
AF | - |
Penicillium chrysogenum |
AFQ | - |
Penicillium chrysogenum |
AFS | - |
Penicillium chrysogenum |
alpha-L-arabinofuranosidase | - |
Penicillium chrysogenum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Penicillium chrysogenum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
stable up to | Penicillium chrysogenum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
14.3 | - |
4-nitrophenyl-alpha-L-arabinofuranoside | enzyme AFQ1, pH 5.0, 37°C | Penicillium chrysogenum | |
59.3 | - |
4-nitrophenyl-alpha-L-arabinofuranoside | enzyme AFS1, pH 5.0, 37°C | Penicillium chrysogenum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.3 | 5 | enzyme AFS1 | Penicillium chrysogenum |
4 | 6.5 | enzyme AFQ1 | Penicillium chrysogenum |