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Literature summary for 3.2.1.55 extracted from

  • Shallom, D.; Belakhov, V.; Solomon, D.; Gilead-Gropper, S.; Baasov, T.; Shoham, G.; Shoham, Y.
    The identification of the acid-base catalyst of alpha-arabinofuranosidase from Geobacillus stearothermophilus T-6, a family 51 glycoside hydrolase (2002), FEBS Lett., 514, 163-167.
    View publication on PubMed

Application

Application Comment Organism
agriculture bioconversion of lignocellulose material for the improvement of animal feedstock digestibility Geobacillus stearothermophilus
nutrition
-
Bacillus subtilis
nutrition aromatic potential in wine, exploitation in flavor improvement and wine aromatization Geobacillus stearothermophilus
paper production
-
Bacillus subtilis
paper production potential industrial use in biobleaching of paper pulp, bioconversion of lignocellulose material to fermentative products and for the improvement of animal feedstock digestibility Geobacillus stearothermophilus

Cloned(Commentary)

Cloned (Comment) Organism
cloning of the abfA T-6 gene, PCR, T7 polymerase expression vector pET9d, expressed in Escherichia coli BL21(DE3) Geobacillus stearothermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
2,5-dinitrophenyl beta-D-xylopyranoside mutant E175A Geobacillus stearothermophilus
0.9
-
3,4-dinitrophenyl beta-D-xylopyranoside mutant E175A Geobacillus stearothermophilus
4.4
-
3,4-dinitrophenyl beta-D-xylopyranoside wild-type Abfa T-6 Geobacillus stearothermophilus
5.3
-
2-nitrophenyl beta-D-xylopyranoside mutant E175A Geobacillus stearothermophilus
8
-
2,5-dinitrophenyl beta-D-xylopyranoside wild-type Abfa T-6 Geobacillus stearothermophilus
15.3
-
2-nitrophenyl beta-D-xylopyranoside wild-type Abfa T-6 Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Agrobacterium tumefaciens
-
-
-
Bacillus subtilis
-
-
-
Geobacillus stearothermophilus
-
T-6
-
Geobacillus stearothermophilus
-
mutant E175A
-
Geobacillus stearothermophilus T-6
-
T-6
-
Halalkalibacterium halodurans
-
-
-
Mesorhizobium loti
-
-
-
Sinorhizobium meliloti
-
-
-
Streptomyces coelicolor
-
-
-
Streptomyces lividans
-
66strain 1326
-
Streptomyces lividans 66 1326
-
66strain 1326
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme, expressed in Escherichia coli BL21(DE3) Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,5-dinitrophenyl beta-D-xylopyranoside + H2O
-
Geobacillus stearothermophilus 2,5-dinitrophenol + beta-D-xylopyranose
-
?
2,5-dinitrophenyl beta-D-xylopyranoside + H2O
-
Geobacillus stearothermophilus T-6 2,5-dinitrophenol + beta-D-xylopyranose
-
?
2-nitrophenyl beta-D-xylopyranoside + H2O
-
Geobacillus stearothermophilus 2-nitrophenol + beta-D-xylopyranose
-
?
2-nitrophenyl beta-D-xylopyranoside + H2O
-
Geobacillus stearothermophilus T-6 2-nitrophenol + beta-D-xylopyranose
-
?
3,4-dinitrophenyl beta-D-xylopyranoside + H2O
-
Geobacillus stearothermophilus 3,4-dinitrophenol + beta-D-xylopyranose
-
?
3,4-dinitrophenyl beta-D-xylopyranoside + H2O
-
Geobacillus stearothermophilus T-6 3,4-dinitrophenol + beta-D-xylopyranose
-
?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Bacillus subtilis p-nitrophenol + L-arabinose
-
?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Geobacillus stearothermophilus p-nitrophenol + L-arabinose
-
?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Streptomyces lividans p-nitrophenol + L-arabinose
-
?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Streptomyces lividans 66 1326 p-nitrophenol + L-arabinose
-
?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Geobacillus stearothermophilus T-6 p-nitrophenol + L-arabinose
-
?
phenyl alpha-L-arabinofuranoside + H2O
-
Sinorhizobium meliloti L-arabinose + phenol
-
ir
phenyl alpha-L-arabinofuranoside + H2O
-
Bacillus subtilis L-arabinose + phenol
-
ir
phenyl alpha-L-arabinofuranoside + H2O
-
Geobacillus stearothermophilus L-arabinose + phenol
-
ir
phenyl alpha-L-arabinofuranoside + H2O
-
Streptomyces coelicolor L-arabinose + phenol
-
ir
phenyl alpha-L-arabinofuranoside + H2O
-
Agrobacterium tumefaciens L-arabinose + phenol
-
ir
phenyl alpha-L-arabinofuranoside + H2O
-
Mesorhizobium loti L-arabinose + phenol
-
ir
phenyl alpha-L-arabinofuranoside + H2O
-
Streptomyces lividans L-arabinose + phenol
-
ir
phenyl alpha-L-arabinofuranoside + H2O
-
Halalkalibacterium halodurans L-arabinose + phenol
-
ir
phenyl alpha-L-arabinofuranoside + H2O
-
Streptomyces lividans 66 1326 L-arabinose + phenol
-
ir
phenyl alpha-L-arabinofuranoside + H2O
-
Geobacillus stearothermophilus T-6 L-arabinose + phenol
-
ir