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Literature summary for 3.2.1.55 extracted from

  • Beylot, M.H.; Emani, K.; McKie, V.A.; Gilbert, H.J.; Pell, G.
    Pseudomonas cellulosa expresses a single membrane-bound glycoside hydrolase family 51 arabinofuranosidase (2001), Biochem. J., 385, 599-605.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
2 genes, abf51A and abf62A, transposon mutagenesis Cellvibrio japonicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7
-
arabinobiose mutant E194A Cellvibrio japonicus

Localization

Localization Comment Organism GeneOntology No. Textmining
outer membrane
-
Cellvibrio japonicus 19867
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
Abf51A, antibody cross reaction, Western blot Cellvibrio japonicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beet arabinan + H2O Geobacillus stearothermophilus
-
L-arabinose + ?
-
?
beet arabinan + H2O Streptomyces chartreusis
-
L-arabinose + ?
-
?
beet arabinan + H2O Cellvibrio japonicus
-
L-arabinose + ?
-
?
wheat L-arabino-D-xylan + H2O Cellvibrio japonicus
-
L-arabinose + ?
-
?

Organism

Organism UniProt Comment Textmining
Cellvibrio japonicus
-
-
-
Geobacillus stearothermophilus
-
-
-
Streptomyces chartreusis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Geobacillus stearothermophilus
-
Streptomyces chartreusis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,5-alpha-L-arabinobiose + H2O
-
Cellvibrio japonicus L-arabinose
-
?
1,5-alpha-L-arabinotriose + 2 H2O
-
Cellvibrio japonicus 3 L-arabinose
-
?
1,5-L-arabinan + H2O
-
Cellvibrio japonicus L-arabinose + ?
-
ir
aryl-alpha-arabinofuranoside + H2O
-
Cellvibrio japonicus L-arabinose + aryl alcohol
-
?
beet arabinan + H2O
-
Geobacillus stearothermophilus L-arabinose + ?
-
?
beet arabinan + H2O
-
Streptomyces chartreusis L-arabinose + ?
-
?
beet arabinan + H2O
-
Cellvibrio japonicus L-arabinose + ?
-
?
L-arabinoxylan + H2O
-
Geobacillus stearothermophilus L-arabinose + ?
-
ir
L-arabinoxylan + H2O
-
Streptomyces chartreusis L-arabinose + ?
-
ir
L-arabinoxylan + H2O preferentially removes alpha-1,2- and alpha-1,3-linked arabinofuranose side chains Cellvibrio japonicus L-arabinose + ?
-
ir
additional information
-
Geobacillus stearothermophilus ?
-
?
additional information does not attack arabinoxylans Streptomyces chartreusis ?
-
?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Geobacillus stearothermophilus p-nitrophenol + L-arabinose
-
?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Streptomyces chartreusis p-nitrophenol + L-arabinose
-
?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Cellvibrio japonicus p-nitrophenol + L-arabinose
-
?
phenyl alpha-L-arabinofuranoside + H2O
-
Geobacillus stearothermophilus L-arabinose + phenol
-
ir
phenyl alpha-L-arabinofuranoside + H2O
-
Streptomyces chartreusis L-arabinose + phenol
-
ir
phenyl alpha-L-arabinofuranoside + H2O
-
Cellvibrio japonicus L-arabinose + phenol
-
ir
wheat L-arabino-D-xylan + H2O
-
Cellvibrio japonicus L-arabinose + ?
-
?