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Literature summary for 3.2.1.55 extracted from
- Genetic and biochemical characterization of a highly thermostable alpha-L-arabinofuranosidase from Thermobacillus xylanilyticus (2000), Appl. Environ. Microbiol., 66, 1734-1736.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Triton X-100 | - |
Thermobacillus xylanilyticus |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Streptomyces lividans |
- |
Thermoclostridium stercorarium |
| arfI and arfII, genes encoding alpha-L-arabinofuranosidase | Bifidobacterium adolescentis |
| gene AbfD3 isolated, inserted into pET24 vector and expressed in Escherichia coli | Thermobacillus xylanilyticus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Aspergillus niger |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | - |
Thermobacillus xylanilyticus | |
| Cu2+ | - |
Aspergillus terreus | |
| Cu2+ | - |
Thermobacillus xylanilyticus | |
| Cu2+ | - |
Thermoclostridium stercorarium | |
| Hg2+ | - |
Aspergillus terreus | |
| Hg2+ | 1 mM HgCl2 inhibits the activity by 98% | Geobacillus stearothermophilus | |
| Hg2+ | - |
Streptomyces purpurascens | |
| Hg2+ | 1 mM HgCl2 inhibits the activity by 98% | Thermobacillus xylanilyticus | |
| Hg2+ | - |
Thermoclostridium stercorarium | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.5 | - |
p-nitrophenyl-alpha-L-arabinose | 75°C | Thermobacillus xylanilyticus | |
| 0.72 | - |
p-nitrophenyl-alpha-L-arabinose | 60°C | Thermobacillus xylanilyticus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56000 | - |
SDS-PAGE | Thermobacillus xylanilyticus |
| 56070 | - |
amino acid sequence analysis | Thermobacillus xylanilyticus |
| 56090 | - |
mass spectrometry | Thermobacillus xylanilyticus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| larchwood xylan + H2O | Thermobacillus xylanilyticus | - |
L-arabinose + ? | - |
? | |
| oat spelt xylan + H2O | Geobacillus stearothermophilus | - |
L-arabinose + ? | - |
? | |
| oat spelt xylan + H2O | Aspergillus niger | - |
L-arabinose + ? | - |
? | |
| oat spelt xylan + H2O | Aspergillus terreus | - |
L-arabinose + ? | - |
? | |
| oat spelt xylan + H2O | Aspergillus awamori | - |
L-arabinose + ? | - |
? | |
| oat spelt xylan + H2O | Thermoclostridium stercorarium | - |
L-arabinose + ? | - |
? | |
| oat spelt xylan + H2O | Thermobacillus xylanilyticus | - |
L-arabinose + ? | - |
? | |
| oat spelt xylan + H2O | Aspergillus awamori IFO 4033 | - |
L-arabinose + ? | - |
? | |
| wheat L-arabino-D-xylan + H2O | Aspergillus niger | - |
L-arabinose + ? | - |
? | |
| wheat L-arabino-D-xylan + H2O | Aspergillus awamori | - |
L-arabinose + ? | - |
? | |
| wheat L-arabino-D-xylan + H2O | Thermobacillus xylanilyticus | - |
L-arabinose + ? | - |
? | |
| wheat L-arabino-D-xylan + H2O | Aspergillus awamori IFO 4033 | - |
L-arabinose + ? | - |
? | |
| wheat L-arabino-D-xylan + H2O | Thermobacillus xylanilyticus D3 | - |
L-arabinose + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus awamori | - |
IFO4033 | - |
| Aspergillus awamori IFO 4033 | - |
IFO4033 | - |
| Aspergillus niger | - |
- |
- |
| Aspergillus terreus | - |
- |
- |
| Aureobasidium pullulans | - |
- |
- |
| Bacillus subtilis | - |
strain 3-6 | - |
| Bacillus subtilis 03. Jun | - |
strain 3-6 | - |
| Bifidobacterium adolescentis | - |
- |
- |
| Breznakibacter xylanolyticus | - |
- |
- |
| Geobacillus stearothermophilus | - |
- |
- |
| Raphanus sativus | - |
radish | - |
| Streptomyces lividans | - |
66strain 1326 | - |
| Streptomyces lividans 66 1326 | - |
66strain 1326 | - |
| Streptomyces purpurascens | - |
IFO 3389 | - |
| Thermobacillus xylanilyticus | - |
D3 | - |
| Thermobacillus xylanilyticus D3 | - |
D3 | - |
| Thermoclostridium stercorarium | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Geobacillus stearothermophilus |
- |
Aspergillus niger |
- |
Bifidobacterium adolescentis |
- |
Streptomyces lividans |
| recombinant protein, expressed in Escherichia coli | Thermobacillus xylanilyticus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | - |
Raphanus sativus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 350 | - |
60°C | Thermobacillus xylanilyticus |
| 490 | - |
75°C | Thermobacillus xylanilyticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-arabinoxylan + H2O | - |
Geobacillus stearothermophilus | L-arabinose + ? | - |
ir | |
| L-arabinoxylan + H2O | - |
Aspergillus niger | L-arabinose + ? | - |
ir | |
| L-arabinoxylan + H2O | - |
Bifidobacterium adolescentis | L-arabinose + ? | - |
ir | |
| L-arabinoxylan + H2O | - |
Streptomyces lividans | L-arabinose + ? | - |
ir | |
| L-arabinoxylan + H2O | - |
Thermoclostridium stercorarium | L-arabinose + ? | - |
ir | |
| L-arabinoxylan + H2O | - |
Thermobacillus xylanilyticus | L-arabinose + ? | - |
ir | |
| L-arabinoxylan + H2O | - |
Streptomyces lividans 66 1326 | L-arabinose + ? | - |
ir | |
| L-arabinoxylan + H2O | - |
Thermobacillus xylanilyticus D3 | L-arabinose + ? | - |
ir | |
| larchwood xylan + H2O | - |
Thermobacillus xylanilyticus | L-arabinose + ? | - |
? | |
| additional information | - |
Bacillus subtilis | ? | - |
? | |
| additional information | - |
Geobacillus stearothermophilus | ? | - |
? | |
| additional information | - |
Aspergillus niger | ? | - |
? | |
| additional information | - |
Aspergillus terreus | ? | - |
? | |
| additional information | - |
Streptomyces lividans | ? | - |
? | |
| additional information | - |
Streptomyces purpurascens | ? | - |
? | |
| additional information | does not hydrolyze p-nitrophenyl-alpha-L-arabinopyranoside or gum arabic | Thermobacillus xylanilyticus | ? | - |
? | |
| additional information | - |
Streptomyces lividans 66 1326 | ? | - |
? | |
| additional information | - |
Bacillus subtilis 03. Jun | ? | - |
? | |
| additional information | does not hydrolyze p-nitrophenyl-alpha-L-arabinopyranoside or gum arabic | Thermobacillus xylanilyticus D3 | ? | - |
? | |
| oat spelt xylan + H2O | - |
Geobacillus stearothermophilus | L-arabinose + ? | - |
? | |
| oat spelt xylan + H2O | - |
Aspergillus niger | L-arabinose + ? | - |
? | |
| oat spelt xylan + H2O | - |
Aspergillus terreus | L-arabinose + ? | - |
? | |
| oat spelt xylan + H2O | - |
Aspergillus awamori | L-arabinose + ? | - |
? | |
| oat spelt xylan + H2O | - |
Thermoclostridium stercorarium | L-arabinose + ? | - |
? | |
| oat spelt xylan + H2O | - |
Thermobacillus xylanilyticus | L-arabinose + ? | - |
? | |
| oat spelt xylan + H2O | - |
Aspergillus awamori IFO 4033 | L-arabinose + ? | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Bacillus subtilis | p-nitrophenol + L-arabinose | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Geobacillus stearothermophilus | p-nitrophenol + L-arabinose | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Aspergillus niger | p-nitrophenol + L-arabinose | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Aspergillus terreus | p-nitrophenol + L-arabinose | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Streptomyces lividans | p-nitrophenol + L-arabinose | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Thermoclostridium stercorarium | p-nitrophenol + L-arabinose | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Streptomyces purpurascens | p-nitrophenol + L-arabinose | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Thermobacillus xylanilyticus | p-nitrophenol + L-arabinose | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Streptomyces lividans 66 1326 | p-nitrophenol + L-arabinose | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Bacillus subtilis 03. Jun | p-nitrophenol + L-arabinose | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Thermobacillus xylanilyticus D3 | p-nitrophenol + L-arabinose | - |
? | |
| p-nitrophenyl-alpha-L-arabinopyranoside + H2O | - |
Thermobacillus xylanilyticus | p-nitrophenol + alpha-L-arabinopyranose | - |
? | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Bacillus subtilis | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Geobacillus stearothermophilus | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Aspergillus niger | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Raphanus sativus | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Bifidobacterium adolescentis | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Aspergillus terreus | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Streptomyces lividans | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Aspergillus awamori | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Thermoclostridium stercorarium | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Aureobasidium pullulans | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Streptomyces purpurascens | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Thermobacillus xylanilyticus | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Breznakibacter xylanolyticus | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Aspergillus awamori IFO 4033 | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Streptomyces lividans 66 1326 | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Bacillus subtilis 03. Jun | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Thermobacillus xylanilyticus D3 | L-arabinose + phenol | - |
ir | |
| wheat L-arabino-D-xylan + H2O | - |
Aspergillus niger | L-arabinose + ? | - |
? | |
| wheat L-arabino-D-xylan + H2O | - |
Aspergillus awamori | L-arabinose + ? | - |
? | |
| wheat L-arabino-D-xylan + H2O | - |
Thermobacillus xylanilyticus | L-arabinose + ? | - |
? | |
| wheat L-arabino-D-xylan + H2O | - |
Aspergillus awamori IFO 4033 | L-arabinose + ? | - |
? | |
| wheat L-arabino-D-xylan + H2O | - |
Thermobacillus xylanilyticus D3 | L-arabinose + ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| arabinoxylan arabinofuranohydrolase | - |
Bifidobacterium adolescentis |
| arabinoxylan arabinofuranohydrolase | - |
Aspergillus awamori |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
- |
Thermobacillus xylanilyticus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
highly stable up to | Thermobacillus xylanilyticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.7 | - |
strain B | Aspergillus niger |
| 5.6 | 6.2 | - |
Thermobacillus xylanilyticus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 11 | - |
Thermobacillus xylanilyticus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 12 | - |
Thermobacillus xylanilyticus |
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